甘丙肽诱导的大鼠生长激素分泌的胆碱能调节

Cholinergic modulation of growth hormone secretion induced by galanin in rats.

作者信息

Tanoh T, Shimatsu A, Murakami Y, Ishikawa Y, Yanaihara N, Imura H

机构信息

Department of Internal Medicine, Kyoto University Faculty of Medicine, Japan.

出版信息

Neuroendocrinology. 1991 Jul;54(1):83-8. doi: 10.1159/000125855.

DOI:10.1159/000125855

PMID:1717869

Abstract

The effects of cholinergic modulation by hexamethonium (HEX), tetraethylammonium (TEA), pirenzepine (PIR) and neostigmine on growth hormone (GH) secretion induced by galanin (GAL) were investigated in awake, freely moving male rats. Intracerebroventricular (i.c.v.) injection of GAL (0.6 nmol) caused a marked increase in plasma GH levels. Pretreatment with HEX (3 or 15 mg/kg BW), TEA (15 mg/kg BW) or PIR (0.5 mg/kg BW) significantly reduced the GAL-induced GH secretion. Pretreatment of animals with neostigmine (0.05 mg/kg BW) or with a specific antisomatostatin antiserum (10 microliters of 1:10 diluted with saline, i.c.v.) reversed the inhibition of GAL-induced GH secretion by HEX. These findings suggest that both nicotinic and muscarinic cholinergic mechanisms interact with the galanin-induced GH secretion by modulating hypothalamic somatostatin release.

摘要

在清醒、自由活动的雄性大鼠中，研究了六甲铵（HEX）、四乙铵（TEA）、哌仑西平（PIR）和新斯的明对甘丙肽（GAL）诱导的生长激素（GH）分泌的胆碱能调节作用。脑室内（i.c.v.）注射GAL（0.6 nmol）导致血浆GH水平显著升高。用HEX（3或15 mg/kg体重）、TEA（15 mg/kg体重）或PIR（0.5 mg/kg体重）预处理可显著降低GAL诱导的GH分泌。用新斯的明（0.05 mg/kg体重）或特异性抗生长抑素抗血清（10微升，用生理盐水按1:10稀释，i.c.v.）预处理动物可逆转HEX对GAL诱导的GH分泌的抑制作用。这些发现表明，烟碱能和毒蕈碱能胆碱能机制均通过调节下丘脑生长抑素释放，与甘丙肽诱导的GH分泌相互作用。