Gur'ianov S A, Kirilina E A, Khaĭdukov S V, Suvorov N I, Molotkovskaia I M, Mikhaĭlova A A
Bioorg Khim. 2006 Nov-Dec;32(6):574-8.
Myelopeptide-4 (MP-4) (Phe-Arg-Pro-Arg-Ile-Met-Thr-Pro), inducing the terminal differentiation of HL-60 leukemia cells, was labeled with fluorescein isothiocyanate. The specific binding of this modified peptide to the surface of HL-60 cells and its ability to penetrate into the cells were studied. It was shown by cytometry and confocal microscopy to be bound on the HL-60 cell surface, to penetrate into their cytoplasm, and finally to concentrate around the cell nucleus. These phenomena are probably necessary for the exhibition of MP-4 differentiating activity.
诱导HL-60白血病细胞终末分化的肌肽-4（MP-4）（苯丙氨酸-精氨酸-脯氨酸-精氨酸-异亮氨酸-甲硫氨酸-苏氨酸-脯氨酸）用异硫氰酸荧光素进行了标记。研究了这种修饰肽与HL-60细胞表面的特异性结合及其穿透细胞的能力。通过细胞术和共聚焦显微镜观察发现，它能结合在HL-60细胞表面，穿透进入细胞质，最终聚集在细胞核周围。这些现象可能是MP-4发挥分化活性所必需的。
