患有动脉粥样硬化的欧洲和南亚男性体内内皮祖细胞数量减少。
Reduced endothelial progenitor cells in European and South Asian men with atherosclerosis.
作者信息
Hughes A D, Coady E, Raynor S, Mayet J, Wright A R, Shore A C, Kooner J S, Thom S A McG, Chaturvedi N
机构信息
Imperial College London, London, UK.
出版信息
Eur J Clin Invest. 2007 Jan;37(1):35-41. doi: 10.1111/j.1365-2362.2007.01743.x.
BACKGROUND
Circulating endothelial progenitor cells (EPCs) play a role in the repair and regeneration of the endothelium and may represent a novel cardiovascular risk factor. South Asian subjects have an increased risk of cardiovascular disease which is not fully explained by known risk factors. This study examined associations of EPCs with atherosclerosis and possible ethnic differences in EPCs.
MATERIALS AND METHODS
A population sample of 58 European and South Asian adult men was enriched with the recruitment of an additional 59 European and South Asian men with known coronary disease. The coronary artery calcification score was measured by multi-slice computerized tomography (CT), carotid and femoral intima-media thickness (IMT), and femoral plaques were measured by ultrasound. The subjects were further subdivided into three categories of coronary artery disease on the basis of coronary artery calcification score and clinical history. Total EPCs and non-senescent EPCs (ns-EPCs) were quantified after 5 days cell culture and the number of late outgrowth colonies was measured over a 6-week test period. Circulating CD34+ haematopoietic precursor cells were measured by flow cytometry.
RESULTS
Individuals with femoral plaques had reduced total and ns-EPCs. The number of ns-EPCs were reduced in individuals with the most coronary atheroma and were inversely related to the coronary calcification score and femoral IMT. These relationships persisted after multivariate adjustment for other risk factors. The numbers of late outgrowth colonies or circulating CD34+ cells were unrelated to the presence of atherosclerosis. There were no differences in the number of EPCs between European and South Asian subjects.
CONCLUSION
The number of EPCs are reduced in subjects with atherosclerosis independent of other risk factors. Reduction in EPC numbers may be an independent risk factor for atherosclerosis but does not explain ethnic differences in cardiovascular risk.
背景
循环内皮祖细胞（EPCs）在内皮修复和再生中发挥作用，可能是一种新的心血管危险因素。南亚人群患心血管疾病的风险增加，而已知的危险因素并不能完全解释这一现象。本研究探讨了EPCs与动脉粥样硬化的关联以及EPCs可能存在的种族差异。
材料与方法
选取58名欧洲和南亚成年男性作为人群样本，另外招募了59名患有冠心病的欧洲和南亚男性。通过多层计算机断层扫描（CT）测量冠状动脉钙化评分，用超声测量颈动脉和股动脉内膜中层厚度（IMT）以及股动脉斑块。根据冠状动脉钙化评分和临床病史，将受试者进一步分为三类冠状动脉疾病。细胞培养5天后对总EPCs和非衰老EPCs（ns-EPCs）进行定量，并在6周的测试期内测量晚期集落形成数量。通过流式细胞术测量循环CD34+造血前体细胞。
结果
有股动脉斑块的个体总EPCs和ns-EPCs减少。冠状动脉粥样硬化最严重的个体中ns-EPCs数量减少，且与冠状动脉钙化评分和股动脉IMT呈负相关。在对其他危险因素进行多变量调整后，这些关系依然存在。晚期集落形成数量或循环CD34+细胞数量与动脉粥样硬化的存在无关。欧洲和南亚受试者之间EPCs数量没有差异。
结论
动脉粥样硬化患者的EPCs数量减少，与其他危险因素无关。EPCs数量减少可能是动脉粥样硬化的独立危险因素，但不能解释心血管风险的种族差异。
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