血小板储存容器的灭菌方法会影响体外参数。

Sterilization method of platelet storage containers affects in vitro parameters.

作者信息

van der Meer P F, Pietersz R N I

机构信息

Sanquin Blood Bank North-West Region, Amsterdam, The Netherlands.

出版信息

Vox Sang. 2007 Jan;92(1):32-6. doi: 10.1111/j.1423-0410.2006.00855.x.

DOI:10.1111/j.1423-0410.2006.00855.x

PMID:17181588

Abstract

BACKGROUND AND AIM

Four butyryl trihexyl phthalate plasticized polyvinyl chloride (BTHC-PVC) containers were compared for storage of leukoreduced platelet concentrates (LR-PC): three ethylene oxide (EtO) sterilized (Gambro, Haemonetics, Fresenius), and one steam-sterilized (Fresenius).

METHODS

LR-PCs were made from 5 buffy coats and 300 ml Composol additive solution, and leukoreduced by filtration. Four LR-PCs were pooled and subsequently divided over the 4 BTHC-PVC bags to prevent donor-dependent differences, and sampled for in vitro analysis on day 1, 2, 5, 7 and 9.

RESULTS

The pH values on day 9 were (mean +/- SD, n = 10): 7.12 +/- 0.03 (Gambro), 7.12 +/- 0.04 (Haemonetics), and 7.07 +/- 0.09 (Fresenius, EtO-sterilized) (not significantly different), vs. 6.91 +/- 0.12 (Fresenius, steam-sterilized; P < 0.001 vs. all EtO-sterilized bags). LR-PCs stored in the steam-sterilized bag exhibited significantly higher glucose consumption and lactate production (P < 0.001 vs. all EtO-sterilized bags).

CONCLUSION

All BTHC-PVC containers allow storage of LR-PCs for up to 9 days with good in vitro parameters. However, the method of sterilization affects the storage conditions of the LR-PCs in these bags.

摘要

背景与目的

比较了四种邻苯二甲酸丁酯三己酯增塑聚氯乙烯（BTHC-PVC）容器用于储存白细胞滤除血小板浓缩物（LR-PC）的情况：三种环氧乙烷（EtO）灭菌的（甘布罗、血液技术公司、费森尤斯），以及一种蒸汽灭菌的（费森尤斯）。

方法

LR-PC由5份 Buffy 层和300 ml Composol添加剂溶液制成，并通过过滤进行白细胞滤除。将四个LR-PC合并，随后分到4个BTHC-PVC袋中以防止供体依赖性差异，并在第1、2、5、7和9天取样进行体外分析。

结果

第9天的pH值（平均值±标准差，n = 10）为：7.12±0.03（甘布罗）、7.12±0.04（血液技术公司）和7.07±0.09（费森尤斯，EtO灭菌）（无显著差异），而6.91±0.12（费森尤斯，蒸汽灭菌；与所有EtO灭菌袋相比，P < 0.001）。储存在蒸汽灭菌袋中的LR-PC表现出显著更高的葡萄糖消耗和乳酸生成（与所有EtO灭菌袋相比，P < 0.001）。