Bashir S, Meli A, Cardigan R
Component Development Laboratory, NHS Blood and Transplant, Cambridge, UK.
Department of Haematology, University of Cambridge, Cambridge, UK.
Transfus Med. 2018 Oct;28(5):380-385. doi: 10.1111/tme.12528. Epub 2018 Apr 11.
Two of the predictive factors of the quality of small volumes of platelets suitable for paediatric use are bag size and material. This study evaluated the storage properties of paediatric platelet aliquots in TOTM-, BTHC- or DINCH-PVC bags.
METHODS/MATERIALS: (i) Three apheresis platelet concentrates (PC) were pooled and split into three units. One was retained as an adult unit (control; polyolefin bag). The second and third units were split into four MacoPharma TOTM-PVC and BTHC-PVC paediatric bags, respectively. (ii) Two apheresis PC were pooled and split into two units. One PC was retained as an adult unit, and the other was split into four Fresenius DINCH-PVC paediatric bags. Testing was performed on storage for pH, blood gases, hypotonic shock response, soluble CD62P, LDH, glucose and lactate, ATP, CD62P, CD63, platelet-derived microparticles and annexin V.
The volumes, platelet yields and pH of all paediatric units met local specifications. The TOTM-PVC bag showed no worse quality than the adult bag up to day 7 for all parameters studied, and it maintained pH higher than BTHC-PVC and DINCH-PVC over storage. The BTHC-PVC bag was shown to be the most gas permeable; however, it had the highest glucose consumption rates and the highest platelet activation.
All bags showed an acceptable in vitro quality. Overall, the TOTM-PVC paediatric bag showed better platelet quality compared to the other storage bags, whereas storage in the BTHC-PVC bag resulted in poorer platelet quality.
适合儿科使用的少量血小板质量的两个预测因素是袋子尺寸和材料。本研究评估了在三（2-乙基己基）偏苯三酸酯（TOTM）袋、丁基化羟基甲苯（BHT）包被的三醋酸纤维素（BTHC）袋或二异壬基环己烷-聚氯乙烯（DINCH-PVC）袋中儿科血小板分装物的储存特性。
方法/材料：（i）将三袋单采血小板浓缩物（PC）合并并分成三个单位。一个作为成人单位保留（对照；聚烯烃袋）。第二个和第三个单位分别分成四个MacoPharma TOTM-PVC和BTHC-PVC儿科袋。（ii）将两袋单采PC合并并分成两个单位。一袋PC作为成人单位保留，另一袋分成四个费森尤斯DINCH-PVC儿科袋。在储存时对pH值、血气、低渗休克反应、可溶性CD62P、乳酸脱氢酶（LDH）、葡萄糖和乳酸、三磷酸腺苷（ATP）、CD62P、CD63、血小板衍生微粒和膜联蛋白V进行检测。
所有儿科单位的体积、血小板产量和pH值均符合当地规格。在研究的所有参数方面，TOTM-PVC袋在第7天之前显示出的质量不比成人袋差，并且在储存过程中其pH值保持高于BTHC-PVC袋和DINCH-PVC袋。BTHC-PVC袋被证明是透气性最强的；然而，它的葡萄糖消耗率最高，血小板活化程度也最高。
所有袋子在体外均显示出可接受的质量。总体而言，与其他储存袋相比，TOTM-PVC儿科袋显示出更好的血小板质量，而储存在BTHC-PVC袋中则导致血小板质量较差。
