从牛颌下粘蛋白中分离出的具有血型A和H活性的中性寡糖的结构表征。
Structural characterization of neutral oligosaccharides with blood-group A and H activity isolated from bovine submaxillary mucin.
作者信息
Savage A V, D'Arcy S M, Donoghue C M
机构信息
Department of Chemistry, University College, Galway, Ireland.
出版信息
Biochem J. 1991 Oct 1;279 ( Pt 1)(Pt 1):95-103. doi: 10.1042/bj2790095.
Abstract
In this study we investigated the structures of 11 neutral oligosaccharides released from bovine submaxillary mucin by alkaline borohydride treatment and isolated by h.p.l.c. One hexa-, one penta-, three tetra-, four tri- and two di-saccharides containing core types 1, 2, 3 or 4 were obtained. We report their structures, determined by a combination of one- and two-dimensional 1H n.m.r. spectroscopy at 270 MHz and methylation analysis involving g.l.c.-m.s., along with their approximate molar ratios. Only three of these oligosaccharides have previously been reported in this source. Of the new oligosaccharides, one contains the blood-group-A antigenic determinant, two contain the blood-group-H type 2 determinant, while another contains the blood-group-H type 3 determinant. The oligosaccharide GlcNAc beta (1----6)[GlcNAc beta (1----3)]GalNAcol, although previously found as a core structure, has been isolated here as a novel trisaccharide.
摘要
在本研究中，我们对通过碱性硼氢化物处理从牛下颌粘液中释放并经高效液相色谱分离得到的11种中性寡糖的结构进行了研究。获得了一种六糖、一种五糖、三种四糖、四种三糖和两种二糖，它们包含核心类型1、2、3或4。我们报告了通过270 MHz的一维和二维¹H核磁共振光谱以及涉及气相色谱-质谱联用的甲基化分析确定的它们的结构，以及它们的近似摩尔比。这些寡糖中只有三种此前曾在该来源中报道过。在新的寡糖中，一种含有血型A抗原决定簇，两种含有血型H 2型决定簇，而另一种含有血型H 3型决定簇。寡糖GlcNAcβ(1→6)[GlcNAcβ(1→3)]GalNAcol虽然此前作为核心结构被发现，但在此处作为一种新型三糖被分离出来。
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