牛下颌粘蛋白的碳水化合物结构
Carbohydrate structures of bovine submaxillary mucin.
作者信息
Tsuji T, Osawa T
出版信息
Carbohydr Res. 1986 Aug 15;151:391-402. doi: 10.1016/s0008-6215(00)90358-6.
The structures of carbohydrate chains derived from bovine submaxillary mucin (BSM) were investigated. Oligosaccharide-alditols released from BSM by alkaline borohydride treatment were separated into three acidic (A-1-A-3) and five neutral (N-1-N-5) oligosaccharide-alditol fractions by liquid chromatography on columns of an ion-exchange resin and Bio-Gel P-4. On the basis of the data obtained on compositional and methylation analyses, and digestion by exoglycosidase, the following structures were assigned to these oligosaccharide-alditols: A-1, alpha-NeuAc (or NeuGc)-(2----6)-GalNAc-ol; A-2, beta-Gal-(1----3)-[alpha-NeuAc-(2----6)]-GalNAc-ol; A-3, beta-GlcNAc-(1----3)-[alpha-NeuAc-(2----6)]-GalNAc-ol; N-1, GalNAc-ol; N-2, beta-Gal-(1----3)-GalNAc-ol; N-3, beta-GlcNAc-(1----3)-GalNAc-ol; N-4, beta-Gal-(1----3)-[beta-GlcNAc-(1----6)]-GalNAc-ol; and N-5, alpha-Fuc-(1----2)-beta-Gal-(1----3)-[beta-GlcNAc-(1----6)]-GalNAc-ol. These results showed the heterogeneous nature of the carbohydrate chains of BSM.
对源自牛下颌粘液素（BSM）的碳水化合物链结构进行了研究。通过碱性硼氢化物处理从BSM释放的低聚糖糖醇，通过离子交换树脂柱和Bio-Gel P-4柱上的液相色谱法，分离为三个酸性（A-1 - A-3）和五个中性（N-1 - N-5）低聚糖糖醇级分。根据在组成和甲基化分析以及外切糖苷酶消化中获得的数据，为这些低聚糖糖醇指定了以下结构：A-1，α-NeuAc（或NeuGc）-(2→6)-GalNAc-ol；A-2，β-Gal-(1→3)-[α-NeuAc-(2→6)]-GalNAc-ol；A-3，β-GlcNAc-(1→3)-[α-NeuAc-(2→6)]-GalNAc-ol；N-1，GalNAc-ol；N-2，β-Gal-(1→3)-GalNAc-ol；N-3，β-GlcNAc-(1→3)-GalNAc-ol；N-4，β-Gal-(1→3)-[β-GlcNAc-(1→6)]-GalNAc-ol；以及N-5，α-Fuc-(1→2)-β-Gal-(1→3)-[β-GlcNAc-(1→6)]-GalNAc-ol。这些结果表明了BSM碳水化合物链的异质性。
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