Kubyshin V L, Gorbach Z V
Ukr Biokhim Zh (1978). 1985 Mar-Apr;57(2):37-41.
Modification of the method for transketolase purification in the rat liver was used to reveal the existence of two molecular forms of this enzyme. One of the forms does not react to development of avitaminosis in animals caused by oxythiamine in different doses. The method is developed for isolation of the basic transketolase form in the rat liver with the 50-80% yield of activity. Km values of two sites for coenzyme binding on protein do not depend on the extent of holoenzyme reconstruction from apoenzyme.
采用改良的大鼠肝脏转酮醇酶纯化方法来揭示该酶的两种分子形式的存在。其中一种形式对不同剂量的氧硫胺素引起的动物维生素缺乏症的发展没有反应。该方法用于分离大鼠肝脏中基本的转酮醇酶形式，活性回收率为50 - 80%。蛋白质上两个辅酶结合位点的Km值不取决于从脱辅酶重建全酶的程度。
