Zereu M, Zettler C G, Cambruzzi E, Zelmanowicz A
Fundação Faculdade de Ciências Médicas de Porto Alegre, Porto Alegre, Brazil.
Gynecol Oncol. 2007 Apr;105(1):172-5. doi: 10.1016/j.ygyno.2006.11.006. Epub 2006 Dec 22.
Adenocarcinoma of the uterine cervix (AC) occurs in 15-20% of primary cervical neoplasias. Although some etiologic factors for squamous cell carcinoma are well defined, and its relationship with sexually transmitted disease as human papillomavirus (HPV) is established, we still do not know about the causative factors of most of AC besides HPV infection.
To determine the presence of herpes simplex virus type 2 (HSV-2) and Chlamydia trachomatis (CT) DNA in AC specimens, and its correlation with HPV infection.
206 paraffin-embedded cases of AC were selected to DNA extraction. The specimens and the DNA were isolated. Samples were first screened for beta-globin DNA sequences, and 67 cases were considered adequate to further analysis. In a previous analysis, DNA of HPV was identified in 79.4% of specimens included in this series (51% HPV 18 and 34% HPV 16). The local ethical committee approved the study.
All samples were negative for HSV-2 DNA and CT DNA.
In our series HSV-2 DNA and CT DNA were not found to be integrated to the genome of adenocarcinoma of the uterine cervix and do not seem to be a co-factor for HPV on the etiology of this histologic subtype.
子宫颈腺癌（AC）占原发性宫颈肿瘤的15%-20%。尽管一些鳞状细胞癌的病因已明确，且其与性传播疾病如人乳头瘤病毒（HPV）的关系也已确立，但除HPV感染外，我们对大多数AC的致病因素仍不了解。
确定AC标本中单纯疱疹病毒2型（HSV-2）和沙眼衣原体（CT）DNA的存在情况及其与HPV感染的相关性。
选取206例AC石蜡包埋病例进行DNA提取。分离标本和DNA。样本首先进行β-珠蛋白DNA序列筛查，67例被认为适合进一步分析。在之前的分析中，本系列中79.4%的标本检测出HPV DNA（51%为HPV 18，34%为HPV 16）。本研究经当地伦理委员会批准。
所有样本的HSV-2 DNA和CT DNA均为阴性。
在我们的研究系列中，未发现HSV-2 DNA和CT DNA整合到子宫颈腺癌基因组中，似乎也不是该组织学亚型病因中HPV的辅助因素。
