Walker M J, Wehland J, Timmis K N, Raupach B, Schmidt M A
Department of Microbiology, GBF-National Research Centre for Biotechnology, Braunschweig, Germany.
Infect Immun. 1991 Nov;59(11):4249-51. doi: 10.1128/iai.59.11.4249-4251.1991.
Three murine monoclonal antibodies (MAb), E19, E205, and E251, raised against pertussis toxin reacted in Western blots (immunoblots) with the S1, S4, and S2-S3 subunits, respectively, and neutralized the Chinese hamster ovary cell-clustering activity of pertussis toxin. MAb E251 recognized a linear synthetic peptide corresponding to amino acids 107 to 120 of the S2 subunit, suggesting a role for this region in receptor binding.
三种针对百日咳毒素产生的鼠单克隆抗体（MAb），即E19、E205和E251，在蛋白质免疫印迹法（免疫印迹）中分别与S1、S4以及S2 - S3亚基发生反应，并中和了百日咳毒素的中国仓鼠卵巢细胞聚集活性。单克隆抗体E251识别一种与S2亚基第107至120位氨基酸相对应的线性合成肽，表明该区域在受体结合中发挥作用。
