Guzman C A, Molinari G, Fountain M W, Rohde M, Timmis K N, Walker M J
Institute of Microbiology, University of Genoa, Italy.
Infect Immun. 1993 Feb;61(2):573-9. doi: 10.1128/iai.61.2.573-579.1993.
Mice were orally vaccinated with liposomes coated with filamentous hemagglutinin (FHA) and detoxified pertussis toxin (PT) of Bordetella pertussis. FHA- and PT-specific immunoglobulin G (IgG) was detected in serum, and both IgG and IgA were detected in lung washes following the immunization. Antibody responses in mice immunized with liposomes coated with FHA and PT were significantly higher than those in mice immunized with free FHA and PT, which demonstrated the adjuvanticity of the liposome carrier. The results indicate the potential usefulness of this approach for eliciting immune responses against FHA and PT (and perhaps other pertussis antigens) in humans and its possible utility in large-scale vaccination to protect against both B. pertussis infection and disease.
用包被有丝状血凝素（FHA）和百日咳博德特氏菌脱毒百日咳毒素（PT）的脂质体对小鼠进行口服免疫。免疫后在血清中检测到FHA和PT特异性免疫球蛋白G（IgG），在肺灌洗液中检测到IgG和IgA。用包被有FHA和PT的脂质体免疫的小鼠的抗体反应明显高于用游离FHA和PT免疫的小鼠，这证明了脂质体载体的佐剂作用。结果表明这种方法在引发人体针对FHA和PT（可能还有其他百日咳抗原）的免疫反应方面具有潜在用途，并且在大规模疫苗接种以预防百日咳博德特氏菌感染和疾病方面可能具有实用性。
