Araujo Ricardo, Pina-Vaz Cidalia, Rodrigues Acacio Gonçalves
Department of Microbiology, Faculty of Medicine, University of Porto, Alameda Prof. Hernani Monteiro, 4200-319 Porto, Portugal.
Int J Antimicrob Agents. 2007 Jan;29(1):108-11. doi: 10.1016/j.ijantimicag.2006.09.019.
The objective of this study was to compare the antifungal susceptibility profiles of 307 environmental strains and 139 clinical isolates of Aspergillus belonging to six different species. Clinical and environmental strains with minimal inhibitory concentrations (MICs) or minimal effective concentrations >or=4microg/mL to amphotericin B (AMB), itraconazole (ITC), voriconazole and caspofungin were seldom detected. However, the susceptibility profile of environmental Aspergillus non-fumigatus strains suggests a native reduced susceptibility of Aspergillus flavus and Aspergillus terreus to AMB. A single environmental strain of Aspergillus nidulans showed high in vitro resistance (MIC>or=16 microg/mL) to ITC. Aspergillus niger showed significantly higher MIC values to ITC compared with the other Aspergillus spp. Conversely, A. fumigatus and Aspergillus glaucus showed higher susceptible profiles to the antifungals. Definition of the breakpoints for the antifungal agents remains urgent. The relationship between the susceptibility pattern and the pathogenic potential also deserves more attention, particularly with regard to non-fumigatus species.
本研究的目的是比较307株环境菌株和139株临床分离株曲霉的抗真菌药敏谱，这些曲霉分属于六个不同的种。很少检测到对两性霉素B（AMB）、伊曲康唑（ITC）、伏立康唑和卡泊芬净的最低抑菌浓度（MIC）或最低有效浓度≥4μg/mL的临床和环境菌株。然而，环境非烟曲霉菌株的药敏谱表明黄曲霉和土曲霉对AMB天然敏感性降低。一株构巢曲霉环境菌株对ITC表现出高体外耐药性（MIC≥16μg/mL）。黑曲霉对ITC的MIC值显著高于其他曲霉属物种。相反，烟曲霉和灰绿曲霉对这些抗真菌药物表现出更高的敏感性。抗真菌药物断点的定义仍然紧迫。药敏模式与致病潜力之间的关系也值得更多关注，特别是对于非烟曲霉物种。
