戊二醛固定对苜蓿花叶病毒免疫原性、颗粒稳定性和抗原反应性的影响以及所引发抗体的特异性。

Effect of glutaraldehyde-fixation on the immunogenicity, particle stability and antigenic reactivity of alfalfa mosaic virus, and the specificity of elicited antibodies.

作者信息

Hajimorad M R, Francki R I

机构信息

Department of Plant Pathology, Waite Agricultural Research Institute, University of Adelaide, Glen Osmond, South Australia.

出版信息

J Virol Methods. 1991 Jun;33(1-2):13-25. doi: 10.1016/0166-0934(91)90003-i.

DOI:10.1016/0166-0934(91)90003-i

PMID:1719013

Abstract

Glutaraldehyde-fixation was shown to stabilize the structural integrity of alfalfa mosaic virus (AMV) particles as well as to increase their immunogenicity and antigenic reactivity. The antigenic reactivity of the particles was substantially increased irrespective of whether the antibodies were from animals immunized with native or fixed AMV, or preparations of coat protein subunits isolated from the virus. No significant changes in the antigenic specificity of AMV particles were detected following glutaraldehyde-fixation. Thus it is possible to raise antisera to AMV with higher titres by using fixed virus as immunogen.

摘要

戊二醛固定已被证明能稳定苜蓿花叶病毒（AMV）颗粒的结构完整性，并提高其免疫原性和抗原反应性。无论抗体是来自用天然或固定化AMV免疫的动物，还是从病毒中分离的外壳蛋白亚基制剂，颗粒的抗原反应性都显著增加。戊二醛固定后未检测到AMV颗粒抗原特异性的显著变化。因此，使用固定化病毒作为免疫原可以产生更高滴度的抗AMV抗血清。