间接双抗体夹心酶联免疫吸附测定法用于检测感染植物上短暂取食后的蚜虫中的苜蓿花叶病毒。

Indirect double antibody sandwich ELISA for detecting alfalfa mosaic virus in aphids after short probes on infected plants.

作者信息

Ahoonmanesh A, Hajimorad M R, Ingham B J, Francki R I

机构信息

Department of Plant Pathology, Waite Agricultural Research Institute, University of Adelaide, Glen Osmond, South Australia.

出版信息

J Virol Methods. 1990 Dec;30(3):271-81. doi: 10.1016/0166-0934(90)90069-r.

DOI:10.1016/0166-0934(90)90069-r

PMID:2086598

Abstract

An indirect double antibody sandwich enzyme-linked immunosorbent assay (IDAS-ELISA) system using antibodies elicited in rabbits and chickens is described for the detection of alfalfa mosaic virus (AMV). The method is capable of detecting 40 pg of homologous purified AMV and was shown to be suitable for detecting the virus in aphids. AMV in the order of 150 pg was detected in single aphids. It was shown that a significant proportion of insects could acquire this or higher amounts of virus during 1 min probes on the leaves of infected plants. The IDAS-ELISA should find applicability in research on the acquisition of viruses by aphids and in epidemiological studies for detecting viruses in insects from traps.

摘要

本文描述了一种间接双抗体夹心酶联免疫吸附测定（IDAS-ELISA）系统，该系统使用兔和鸡体内产生的抗体来检测苜蓿花叶病毒（AMV）。该方法能够检测到40 pg的同源纯化AMV，并且被证明适用于检测蚜虫体内的病毒。在单个蚜虫中检测到了约150 pg的AMV。结果表明，相当一部分昆虫在受感染植物叶片上1分钟的取食过程中能够获得这种或更高数量的病毒。IDAS-ELISA在蚜虫获取病毒的研究以及检测诱捕昆虫中病毒的流行病学研究中应具有适用性。