Dekker E L, Dore I, Porta C, van Regenmortel M H
Arch Virol. 1987;94(3-4):191-203. doi: 10.1007/BF01310713.
Ten monoclonal antibodies (McAbs) were raised to the tobamovirus, tomato mosaic virus (ToMV). Seven of the McAbs reacted with ToMV only when tested in an ELISA format, involving antibody-coated plates, which preserved the quaternary conformation of the viral protein. Two McAbs which reacted with ToMV only in an ELISA using virus-coated plates recognized an antigenic determinant present only on the disassembled form of the viral coat protein. One McAb reacted with both the assembled and dissociated forms of ToMV protein. All McAbs reacted in an identical manner with the 15 ToMV strains and isolates tested, confirming the previously established serological homogeneity of the ToMV species. Only two of the McAbs cross-reacted with two other tobamoviruses: tobacco mosaic virus (TMV) and ribgrass mosaic virus. Seven variations of ELISA were compared for their suitability to detect ToMV and TMV in tomato plants. Double antibody sandwich ELISA could be performed with a single McAb to ToMV as enough accessible epitopes are present on the virus to bind simultaneously to the coating and biotin-labelled antibody.
制备了针对烟草花叶病毒属病毒——番茄花叶病毒（ToMV）的十种单克隆抗体（McAbs）。其中七种单克隆抗体仅在以酶联免疫吸附测定（ELISA）形式进行检测时与ToMV发生反应，该检测涉及抗体包被板，可保留病毒蛋白的四级结构。两种单克隆抗体仅在使用病毒包被板的ELISA中与ToMV发生反应，它们识别仅存在于病毒衣壳蛋白解离形式上的一个抗原决定簇。一种单克隆抗体与ToMV蛋白的组装形式和解离形式均发生反应。所有单克隆抗体与所检测的15种ToMV菌株和分离株反应方式相同，证实了之前确定的ToMV种的血清学同质性。只有两种单克隆抗体与另外两种烟草花叶病毒属病毒发生交叉反应：烟草花叶病毒（TMV）和长叶车前花叶病毒。比较了七种ELISA变体检测番茄植株中ToMV和TMV的适用性。由于病毒上存在足够多可及的表位，能够同时与包被抗体和生物素标记抗体结合，因此可以用一种针对ToMV的单克隆抗体进行双抗体夹心ELISA。
