Sun Hongxiang, Ye Yiping, Pan Yuanjian
College of Animal Science, Zhejiang University, Hangzhou 310029, PR China.
Chem Biodivers. 2005 Apr;2(4):510-5. doi: 10.1002/cbdv.200590032.
The total saponin extract from the dried roots of Panax notoginseng (Burk.) F. H. Chen possesses immunological-adjuvant activities. Guided by in vivo immunological tests, further study on this fraction afforded three active dammarane-type saponins. Their structures were determined on the basis of chemical evidence and extensive spectroscopic methods, including 1D- and 2D-NMR. The novel compound (20S)-protopanaxatriol 20-O-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranoside (1), and the two known compounds ginsenoside Rh4 (2) and notoginsenoside K (3) exhibited immunological-adjuvant activities on the humoral immune responses of ICR mice against ovalbumin (OVA).
三七干燥根中的总皂苷提取物具有免疫佐剂活性。在体内免疫试验的指导下，对该提取物进行了进一步研究，得到了三种具有活性的达玛烷型皂苷。通过化学证据和包括一维和二维核磁共振在内的广泛光谱方法确定了它们的结构。新型化合物（20S）-原人参三醇20-O-β-D-吡喃葡萄糖基-（1→6）-β-D-吡喃葡萄糖苷（1）以及两种已知化合物人参皂苷Rh4（2）和三七皂苷K（3）对ICR小鼠针对卵清蛋白（OVA）的体液免疫反应表现出免疫佐剂活性。
