Center for Pharmacological Research, State Key Laboratory of Generic Manufacture Technology of Chinese Traditional Medicine, Lunan Pharmaceutical Group Co., Ltd., Linyi, China.
Department of Traditional Chinese Medicine, Qilu Hospital of Shandong University, Jinan, China.
Front Immunol. 2022 Oct 25;13:983501. doi: 10.3389/fimmu.2022.983501. eCollection 2022.
With the rapid aging of the population, the control of age-related disease susceptibility and prognosis faces greater challenges. There is an urgent need for a strategy to maintain the vitality of elderly people. In this study, the effect of Renshen Guben (RSGB) oral liquid was investigated on an accelerated aging mice model of thyrotoxicosis by conventional detection methods combined with multiomics technology. The results showed that RSGB increased the number of neutrophils and lymphocytes, enhanced the function of lymphocytes, and increased the levels of complement and antimicrobial peptides, which indicated that RSGB improved the immunity of thyrotoxicosis mice at the cellular and molecular levels. RSGB corrected malnutrition in thyrotoxicosis mice by improving anemia, hypoalbuminemia, ion transporters, and vitamin-binding proteins. RSGB significantly reduced the lipotoxicity by reducing the level of fatty acids, triglyceride, sphingolipids, and glucocorticoids, thus increasing the level of docosapentaenoic acid (DPA) and bile acids, which contributed to improve immunosenescence. The intestinal defense ability of thyrotoxicosis mice was enhanced with the increase of bile acids and lactic acid bacteria by the RSGB treatment. The plant metabolomics analysis showed that there were various active components in RSGB oral liquid and medicated serum, including terpenoids, phenolic acids, flavonoids, tannin, alkaloids, organic acids, phenolamines, amino acids, and others. They have antioxidant, immune regulation, and anti-aging effects, which was the material basis of RSGB. Totally, RSGB protected the thyrotoxicosis mice against aging by improving immunosenescence, hypoproteinemia, lipotoxicity, and the intestinal flora. It will be beneficial for improving the disease susceptibility and prognosis of the elderly.
随着人口老龄化的快速发展，控制与年龄相关的疾病易感性和预后面临更大的挑战。迫切需要制定一种策略来保持老年人的活力。在这项研究中，通过常规检测方法结合多组学技术，研究了人参固本口服液对甲状腺机能亢进加速衰老小鼠模型的影响。结果表明，人参固本口服液增加了中性粒细胞和淋巴细胞的数量，增强了淋巴细胞的功能，提高了补体和抗菌肽的水平，这表明人参固本口服液在细胞和分子水平上改善了甲状腺机能亢进小鼠的免疫力。人参固本口服液通过改善贫血、低蛋白血症、离子转运体和维生素结合蛋白纠正甲状腺机能亢进小鼠的营养不良。人参固本口服液通过降低脂肪酸、甘油三酯、鞘脂和糖皮质激素的水平，显著降低脂毒性，从而增加二十二碳五烯酸（DPA）和胆汁酸的水平，有助于改善免疫衰老。人参固本口服液治疗后，甲状腺机能亢进小鼠的肠道防御能力增强，胆汁酸和乳酸菌增加。植物代谢组学分析表明，人参固本口服液和含药血清中含有各种活性成分，包括萜类、酚酸、类黄酮、单宁、生物碱、有机酸、苯乙胺、氨基酸等。它们具有抗氧化、免疫调节和抗衰老作用，这是人参固本口服液的物质基础。总的来说，人参固本口服液通过改善免疫衰老、低蛋白血症、脂毒性和肠道菌群，保护甲状腺机能亢进小鼠免受衰老的影响。这将有利于改善老年人的疾病易感性和预后。
