来自滇黄精的细胞毒性甾体皂苷。

Cytotoxic steroidal saponins from Polygonatum punctatum.

作者信息

Yang Qing-Xiong, Yang Chong-Ren

机构信息

College of Biotechnology and Bioengineering, Guizhou Normal University, 116 Baoshan North Road, Guiyang, 550001, PR China.

出版信息

Chem Biodivers. 2006 Dec;3(12):1349-55. doi: 10.1002/cbdv.200690137.

DOI:10.1002/cbdv.200690137

PMID:17193248

Abstract

Four new steroidal saponins, polypunctosides A-D (1-4, resp.), were isolated from the rhizomes of Polygonatum punctatum, together with five known steroidal saponins. On the basis of chemical and spectral evidence, the structures of the new saponins were established as (3beta,23S,25R)-23-(alpha-L-arabinopyranosyloxy)spirost-5-en-3-yl 4-O-(6-deoxy-alpha-L-mannopyranosyl)-D-glucopyranoside (1), (3beta,23S,25R)-23-[(2-O-acetyl-alpha-L-arabinopyranosyl)oxy]spirost-5-en-3-yl 4-O-(6-deoxy-alpha-L-mannopyranosyl)-D-glucopyranoside (2), (3beta,23S,25R)-23-[(3-O-acetyl-alpha-L-arabinopyranosyl)oxy]spirost-5-en-3-yl 4-O-(6-deoxy-alpha-L-mannopyranosyl)-D-glucopyranoside (3), and (3beta,23S,25R)-23-[(4-O-acetyl-alpha-L-arabinopyranosyl)oxy]spirost-5-en-3-yl 4-O-(6-deoxy-alpha-L-mannopyranosyl)-D-glucopyranoside (4). The cytotoxic activity of the isolated saponins was evaluated towards HeLa cells.

摘要

从滇黄精根茎中分离得到4个新的甾体皂苷，即多花黄精苷A - D（分别为1 - 4），以及5个已知的甾体皂苷。基于化学和光谱证据，确定新皂苷的结构为(3β,23S,25R)-23-(α-L-阿拉伯吡喃糖氧基)螺旋甾-5-烯-3-基4-O-(6-脱氧-α-L-甘露吡喃糖基)-D-葡萄糖苷（1）、(3β,23S,25R)-23-[(2-O-乙酰基-α-L-阿拉伯吡喃糖基)氧基]螺旋甾-5-烯-3-基4-O-(6-脱氧-α-L-甘露吡喃糖基)-D-葡萄糖苷（2）、(3β,23S,25R)-23-[(3-O-乙酰基-α-L-阿拉伯吡喃糖基)氧基]螺旋甾-5-烯-3-基4-O-(6-脱氧-α-L-甘露吡喃糖基)-D-葡萄糖苷（3）和(3β,23S,25R)-23-[(4-O-乙酰基-α-L-阿拉伯吡喃糖基)氧基]螺旋甾-5-烯-3-基4-O-(6-脱氧-α-L-甘露吡喃糖基)-D-葡萄糖苷（4）。对分离得到的皂苷针对人宫颈癌HeLa细胞的细胞毒活性进行了评价。