Hur Yun-Gyoung, Suh Chang-Hee, Kim Sungjoo, Won Jonghwa
Division of Immune Regulation, Mogam Biotechnology Research Institute, Yongin City, Giheung-gu, Gyounggi-do, South Korea.
J Clin Immunol. 2007 Jan;27(1):36-45. doi: 10.1007/s10875-006-9057-8. Epub 2006 Dec 29.
T cells play an important role in the initiation and the progression of rheumatoid arthritis (RA) and depletion of potentially pathogenic T cells was suggested as an important therapeutic protocol. We determined if rosmarinic acid (RosA), known as a secondary metabolite from herbal plants, had apoptotic activity toward T cells from RA patients and further verified target T-cell subsets. CD3(+)CD25(+) activated T-cell subsets from most of the RA patients displayed significantly higher apoptosis rates than did the PBMCs and total CD3(+) T cells. Furthermore, activated and effector CD4(+) T cells, including CD4(+)CD25(+) and CD4(+)CD45RO(+) T cells, had a tendency of being more susceptible to RosA-induced apoptosis than that of resting and naïve T-cell subsets. RosA induced the release of cytochrome c from mitochondria and the blockage of mitochondrial depolarization inhibited apoptosis. Taken together, these results suggest that RosA induces apoptosis of activated T-cell subsets from RA patients via a mitochondrial pathway.
T细胞在类风湿关节炎（RA）的发病和进展中起重要作用，清除潜在致病性T细胞被认为是一种重要的治疗方案。我们研究了迷迭香酸（RosA）这种已知的草本植物次生代谢产物，是否对RA患者的T细胞具有凋亡活性，并进一步验证了目标T细胞亚群。大多数RA患者的CD3(+)CD25(+)活化T细胞亚群的凋亡率显著高于外周血单个核细胞（PBMCs）和总CD3(+) T细胞。此外，活化的效应CD4(+) T细胞，包括CD4(+)CD25(+)和CD4(+)CD45RO(+) T细胞，比静止和初始T细胞亚群更容易受到RosA诱导的凋亡影响。RosA诱导线粒体释放细胞色素c，线粒体去极化的阻断抑制凋亡。综上所述，这些结果表明RosA通过线粒体途径诱导RA患者活化T细胞亚群的凋亡。
