Blumberg Sara, Schroeder Mariana, Malkesman Oz, Torregrossa Ann Marie, Smith Gerard P, Weller Aron
Developmental Psychobiology Laboratory, Department of Psychology and the Gonda (Goldschmeid) Brain Research Center, Bar Ilan University, Ramat Gan 52900, Israel, and Department of Psychiatry, New York-Presbyterian Hospital, White Plains, NY 10605, USA.
Brain Res. 2007 Feb 23;1134(1):140-7. doi: 10.1016/j.brainres.2006.11.062. Epub 2006 Dec 28.
Equivolumetric gastric preloads of corn oil and mineral oil administered to rats on postnatal day 12 (P12) inhibited intake equally during a 30-min test of independent ingestion (II), but preloads of corn oil inhibited intake significantly more than preloads of mineral oil on P15 and P18 [Weller, A., Gispan, I.H., Armony-Sivan, R., Ritter, R.C., Smith, G.P., 1997. Preloads of corn oil inhibit independent ingestion on postnatal day 15 in rats. Physiol. Behav. 62, 871-874]. It is possible that the equivalent inhibition of intake by the oil preloads on P12 resulted from the failure of the preabsorptive sensory properties of the preloads to be discriminated by peripheral or central sensory mechanisms. To investigate this possibility, we administered equivolumetric gastric preloads of 25% corn oil and 25% mineral oil to pups on P9-12 and counted the number of c-Fos-like immunoreactive (CFLI) cells in central sites that are activated by food intake and postingestive preabsortive mechanisms in adult rats and in pups on P10-11. The major result was that preloads of 25% corn oil and 25% mineral oil that produced equivalent inhibition of II intake produced differential increases of CFLI cells in the forebrain and hindbrain. Specifically, preloads of corn oil increased the number of CFLI cells in the caudal Nucleus Tractus Solitarius significantly more than preloads of mineral oil. Furthermore, preloads of corn oil increased the number of CFLI cells in the Paraventricular and Supraoptic nuclei, but preloads of mineral oil did not. This differential pattern of increases of CFLI cells is evidence that the brain discriminates the preabsorptive sensory properties of preloads of corn oil and mineral oil on P9-12.
在出生后第12天（P12）给大鼠等量的玉米油和矿物油进行胃预负荷，在30分钟的自主摄食（II）测试中，二者对摄食的抑制作用相同，但在P15和P18时，玉米油预负荷对摄食的抑制作用明显大于矿物油预负荷[韦勒，A.，吉斯潘，I.H.，阿蒙尼 - 西万，R.，里特，R.C.，史密斯，G.P.，1997年。玉米油预负荷抑制出生后第15天大鼠的自主摄食。生理学与行为。62，871 - 874]。P12时油预负荷对摄食的等效抑制作用可能是由于预负荷的吸收前感觉特性未能被外周或中枢感觉机制区分所致。为了研究这种可能性，我们在P9 - 12给幼崽等量的25%玉米油和25%矿物油进行胃预负荷，并计数成年大鼠和P10 - 11幼崽中由食物摄入和吸收后吸收前机制激活的中枢部位中类c - Fos免疫反应性（CFLI）细胞的数量。主要结果是，对II摄食产生等效抑制作用的25%玉米油和25%矿物油预负荷在前脑和后脑产生了不同程度的CFLI细胞增加。具体而言，玉米油预负荷使尾侧孤束核中CFLI细胞的数量增加显著多于矿物油预负荷。此外，玉米油预负荷增加了室旁核和视上核中CFLI细胞的数量，但矿物油预负荷没有。CFLI细胞增加的这种差异模式证明，大脑在P9 - 12时能够区分玉米油和矿物油预负荷的吸收前感觉特性。
