Kim Dong Hyun, Jeon Su Jin, Son Kun Ho, Jung Ji Wook, Lee Seungjoo, Yoon Byung Hoon, Lee Jong-Ju, Cho Young-Wuk, Cheong Jae Hoon, Ko Kwang Ho, Ryu Jong Hoon
Department of Oriental Pharmaceutical Science and Kyung Hee East-West Pharmaceutical Research Institute, College of Pharmacy, Kyung Hee University, Dongdaemoon-Ku, Seoul, Korea.
Neurobiol Learn Mem. 2007 May;87(4):536-46. doi: 10.1016/j.nlm.2006.11.005. Epub 2006 Dec 28.
Oroxylin A is a flavonoid and was originally isolated from the root of Scutellaria baicalensis Georgi., one of the most important medicinal herbs in traditional Chinese medicine. The aim of this study was to investigate the ameliorating effects of oroxylin A on memory impairment using the passive avoidance test, the Y-maze test, and the Morris water maze test in mice. Drug-induced amnesia was induced by administering scopolamine (1 mg/kg, i.p.) or diazepam (1 mg/kg, i.p.). Oroxylin A (5 mg/kg) significantly reversed cognitive impairments in mice by passive avoidance and the Y-maze testing (P<.05). Oroxylin A also improved escape latencies in training trials and increased swimming times and distances within the target zone of the Morris water maze (P<.05). Moreover, the ameliorating effects of oroxylin A were antagonized by both muscimol and diazepam (0.25 mg/kg, i.p., respectively), which are GABA(A) receptor agonists. Furthermore, oroxylin A (100 microM) was found to inhibit GABA-induced inward Cl(-) current in a single cortical neuron. These results suggest that oroxylin A may be useful for the treatment of cognitive impairments induced by cholinergic dysfunction via the GABAergic nervous system.
木犀草素A是一种黄酮类化合物，最初从黄芩（传统中药中最重要的草药之一）的根部分离得到。本研究的目的是使用被动回避试验、Y迷宫试验和莫里斯水迷宫试验，研究木犀草素A对小鼠记忆障碍的改善作用。通过腹腔注射东莨菪碱（1mg/kg）或地西泮（1mg/kg）诱导药物性失忆。木犀草素A（5mg/kg）通过被动回避和Y迷宫试验显著逆转了小鼠的认知障碍（P<0.05）。木犀草素A还改善了训练试验中的逃避潜伏期，并增加了莫里斯水迷宫目标区域内的游泳时间和距离（P<0.05）。此外，木犀草素A的改善作用被GABA(A)受体激动剂麝香醇和地西泮（分别为0.25mg/kg，腹腔注射）拮抗。此外，发现木犀草素A（100μM）可抑制单个皮质神经元中GABA诱导的内向Cl(-)电流。这些结果表明，木犀草素A可能对治疗由胆碱能功能障碍通过GABA能神经系统引起的认知障碍有用。
