Rebiere Hervé, Mazel Bernard, Civade Corinne, Bonnet Pierre-Antoine
Agence Française de Sécurité Sanitaire des Produits de Santé, Direction des Laboratoires et des Contrôles, Unité Physico Chimie 2, 635 rue de la Garenne, 34740 Vendargues, France.
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 May 1;850(1-2):376-83. doi: 10.1016/j.jchromb.2006.12.007. Epub 2006 Dec 27.
Three classes of antiretroviral agents are usually available for the treatment of HIV infection: nucleoside reverse transcriptase inhibitors (IN), non-nucleoside reverse transcriptase inhibitors (INN) and protease inhibitors (IP). Two methods by reversed-phase liquid chromatography were developed for the analysis of 19 antiretroviral molecules belonging to these three therapeutic classes and used in medicinal products. Both of these HPLC techniques use a C18 column and UV detection. The first method is for IN family analysis and allows eight molecules to be separated: zalcitabine, lamivudine, amdoxovir, emtricitabine, didanosine, stavudine, zidovudine and abacavir. The second method is for INN and IP family analysis and allows 11 molecules to be separated: fosamprenavir, nevirapine, indinavir, amprenavir, saquinavir, atazanavir, ritonavir, lopinavir, efavirenz, nelfinavir and tipranavir. The combination of these two methods makes possible the quality control of mono-, bi- or tri-therapy pharmaceutical products and the detection of illegal products sold particularly in developing countries.
通常有三类抗逆转录病毒药物可用于治疗HIV感染：核苷类逆转录酶抑制剂（IN）、非核苷类逆转录酶抑制剂（INN）和蛋白酶抑制剂（IP）。开发了两种反相液相色谱法，用于分析属于这三种治疗类别并用于药品中的19种抗逆转录病毒分子。这两种高效液相色谱技术均使用C18柱和紫外检测。第一种方法用于分析IN类药物，可分离出8种分子：扎西他滨、拉米夫定、安多昔韦、恩曲他滨、去羟肌苷、司他夫定、齐多夫定和阿巴卡韦。第二种方法用于分析INN类和IP类药物，可分离出11种分子：福沙普那韦、奈韦拉平、茚地那韦、安普那韦、沙奎那韦、阿他扎那韦、利托那韦、洛匹那韦、依非韦伦、奈非那韦和替拉那韦。这两种方法的结合使得对单一疗法、双疗法或三疗法药品进行质量控制以及检测特别是在发展中国家销售的非法药品成为可能。
