Fallahi-Sichani Mohammad, Soleimani Masoud, Najafi Seyed Mahmoud Arab, Kiani Jafar, Arefian Ehsan, Atashi Amir
Department of Biotechnology, University College of Science, University of Tehran, Tehran 14155-6455, Iran.
Cell Biol Int. 2007 Mar;31(3):299-303. doi: 10.1016/j.cellbi.2006.11.011. Epub 2006 Nov 19.
An intensive study is underway to evaluate different potential candidates for cell therapy of neurodegenerative disorders such as Parkinson's disease (PD). Availability and lower immunogenicity compared to other sources for stem cell therapy such as bone marrow have made human umbilical cord blood stem cells a considerable source for cell therapy. The present study aimed to investigate differentiation of recently introduced pluripotent cord blood stem cells, known as unrestricted somatic stem cells (USSCs), into cells with neural features in serum-withdrawal medium. Using reverse transcription polymerase chain reaction and immunocytochemistry assays, we have shown the expression of neuron-specific genes following a 2week treatment of USSCs in serum-withdrawal induction medium. In addition, we have found that USSCs and USSC-derived neuron-like cells express transcripts of genes associated with development and/or survival of dopaminergic mesencephalic neurons including En1, En2, Nurr1, Ptx3, Pax2, Wnt1 and Wnt3a. The expression of dopamine-associated genes suggests that these cells may be potential candidates to be used for cell therapy of PD.
一项深入研究正在进行，以评估用于神经退行性疾病如帕金森病（PD）细胞治疗的不同潜在候选者。与骨髓等其他干细胞治疗来源相比，人脐带血干细胞的可得性及较低的免疫原性使其成为细胞治疗的重要来源。本研究旨在探讨最近引入的多能脐带血干细胞，即无限制体细胞干细胞（USSCs），在无血清培养基中向具有神经特征的细胞分化的情况。通过逆转录聚合酶链反应和免疫细胞化学分析，我们发现，在无血清诱导培养基中对USSCs进行2周处理后，神经元特异性基因有表达。此外，我们还发现USSCs和USSC衍生的神经元样细胞表达与多巴胺能中脑神经元发育和/或存活相关的基因转录本，包括En1、En2、Nurr1、Ptx3、Pax2、Wnt1和Wnt3a。多巴胺相关基因的表达表明，这些细胞可能是用于PD细胞治疗的潜在候选者。
