Kögler Gesine, Sensken Sandra, Wernet Peter
Institute for Transplantation Diagnostics and Cell Therapeutics, Heinrich-Heine-University Medical Center, Düsseldorf, Germany.
Exp Hematol. 2006 Nov;34(11):1589-95. doi: 10.1016/j.exphem.2006.07.011.
Generation and expansion of pluripotent unrestricted somatic stem cells (USSCs) and mesenchymal cells (MSCs) from human cord blood as well their functional characterization were assessed.
USSC generation was initiated from fresh and cryopreserved cord blood (CB). Culture conditions and functional assays distinguishing between USSCs and MSCs (e.g., endodermal differentiation) are described.
USSC cultures were initiated from 573 placental CB samples with a total generation frequency of 35.4% (n = 203), whereas successful generation of USSCs from cryopreserved products was scare. Medium, dexamethasone, and the fetal calf serum source are the major parameters to generate and expand the cells in a pluripotent state (osteogeneic, neural, and endodermal differentiation).
USSC or MSC cultures can be easily obtained from fresh CB samples, good manufacturing practice production of such cells is feasible. These USSCs or MSCs from CB, when matched for HLA antigens, may serve as an allogeneic stem cell source for the development of cellular therapy for tissue repair.
评估从人脐带血中生成和扩增多能性无限制体细胞干细胞（USSCs）和间充质细胞（MSCs）及其功能特性。
从新鲜和冷冻保存的脐带血（CB）中开始生成USSCs。描述了区分USSCs和MSCs的培养条件和功能测定（例如内胚层分化）。
从573份胎盘CB样本中开始进行USSC培养，总生成频率为35.4%（n = 203），而从冷冻保存产物中成功生成USSCs的情况很少。培养基、地塞米松和胎牛血清来源是在多能状态下（成骨、神经和内胚层分化）生成和扩增细胞的主要参数。
USSC或MSC培养物可轻松从新鲜CB样本中获得，以良好生产规范生产此类细胞是可行的。这些来自CB的USSCs或MSCs，当与HLA抗原匹配时，可作为用于组织修复细胞治疗开发的同种异体干细胞来源。
