Zákovská Alena, Capková Lucie, Serý Omar, Halouzka Jirí, Dendis Milos
Department of Comparative Animal Physiology and General Zoology, Faculty of Science, Masaryk University, Kotlarska 2, CZ-611 37 Brno, Czech Republic.
Ann Agric Environ Med. 2006;13(2):345-8.
A total of 662 samples (winter period: 469; summer period: 193 specimens) of female mosquitoes of the genus Culex, Aedes and Anopheles were collected during the period March 2000-April 2001 from the locality of Vysoke Myto (Eastern Bohemia, Czech Republic). They were examined by dark field microscopy for the presence of spirochetes. The motile spirochetes were observed in 4.2 % of all species of investigated mosquitoes. One spirochetal strain out of the 8 isolation attempts (BRZ14) was obtained (cultivation rate was 12.5 %) and the spirochetal strain was then successfully cultivated and identified using PCR for the presence of Borrelia burgdorferi s.l., and subsequently with the RFLP as genomospecies Borrelia afzelii. This strain was derived from overwintering Culex (Culex) pipiens biotype molestus female mosquitoe. This is apparently one of the sporadic cases of the occurrence of pathogenic borreliae in haematophagous arthropods, other than Ixodes ricinus complex ticks.
2000年3月至2001年4月期间，从捷克共和国东波希米亚的维索基米托采集了总共662份库蚊属、伊蚊属和按蚊属雌性蚊子样本（冬季：469份；夏季：193份）。通过暗视野显微镜检查是否存在螺旋体。在所调查的所有蚊子种类中，有4.2%观察到活动的螺旋体。在8次分离尝试中有1株螺旋体菌株（BRZ14）获得成功（培养率为12.5%），随后该螺旋体菌株成功培养，并通过聚合酶链反应检测是否存在伯氏疏螺旋体复合群，随后用限制性片段长度多态性鉴定为阿氏疏螺旋体基因组种。该菌株源自越冬的尖音库蚊（库蚊）致倦亚种雌性蚊子。这显然是除蓖麻硬蜱复合体外，在吸血节肢动物中出现致病性疏螺旋体的散发病例之一。
