Giamarellos-Bourboulis E J, Antonopoulou A, Petropoulou C, Mouktaroudi M, Spyridaki E, Baziaka F, Pelekanou A, Giamarellou H, Stavrianeas N G
4th Department of Internal Medicine, Univeraity of Athens, Medical School, University General Hospital Attikon, 1 Rimini Street, 124 64 Athens, Greece.
Br J Dermatol. 2007 Jan;156(1):51-6. doi: 10.1111/j.1365-2133.2006.07556.x.
The clinical improvement of hidradenitis suppurativa reported in a small number of patients with antitumour necrosis factor (anti-TNF)-alpha therapies supports the hypothesis for an altered immune response in these patients.
To evaluate the state of the innate and adaptive immune responses in patients with hidradenitis suppurativa.
Fifty-three patients and six healthy controls were studied. Blood was sampled and subpopulations of lymphocytes were analysed by flow cytometry; monocytes were isolated and their function was evaluated from the concentrations of TNF-alpha and interleukin (IL)-6 in supernatants of cell cultures after triggering with endotoxins (lipopolysaccharides). TNF-alpha and IL-6 were estimated by an enzyme immunoassay.
CD3/CD8 lymphocytes were lower in patients with involvement of the perineum than in controls; patients with involvement of the breast had higher levels of natural killer (NK) cells than controls. A negative correlation was found between years lapsing since initial presentation of lesions of hidradenitis and the percentage of NK cells. Monocytes isolated from healthy volunteers were more active for the secretion of TNF-alpha and IL-6 than those of patients with hidradenitis suppurativa.
A reduction in the percentage of NK cells over time and a lower monocyte response to triggering by bacterial components is observed in patients with hidradenitis suppurativa. Further research is needed to clarify if these changes are connected to an autoimmune mechanism in the pathogenesis of hidradenitis suppurativa.
少数接受抗肿瘤坏死因子（抗TNF）-α治疗的化脓性汗腺炎患者出现临床改善，这支持了这些患者免疫反应改变的假说。
评估化脓性汗腺炎患者先天性和适应性免疫反应的状态。
对53例患者和6名健康对照者进行研究。采集血液，通过流式细胞术分析淋巴细胞亚群；分离单核细胞，并根据内毒素（脂多糖）刺激后细胞培养上清液中TNF-α和白细胞介素（IL）-6的浓度评估其功能。通过酶免疫测定法估算TNF-α和IL-6。
会阴部受累的患者CD3/CD8淋巴细胞低于对照组；乳房受累的患者自然杀伤（NK）细胞水平高于对照组。化脓性汗腺炎病变初次出现后的时间与NK细胞百分比之间呈负相关。从健康志愿者分离的单核细胞比化脓性汗腺炎患者的单核细胞分泌TNF-α和IL-6更活跃。
化脓性汗腺炎患者中观察到NK细胞百分比随时间减少，且单核细胞对细菌成分刺激的反应较低。需要进一步研究以阐明这些变化是否与化脓性汗腺炎发病机制中的自身免疫机制有关。
