家栖红猎蝽、饰边红猎蝽、长红猎蝽和斯氏红猎蝽核糖体DNA的ITS-2区域多态性
Polymorphism of the ITS-2 region of the ribosomal DNA of the Triatominae Rhodnius domesticus, R. pictipes, R. prolixus and R. stali.
作者信息
Naegele M P, Da Costa P I, Da Rosa J A
机构信息
Departamento de Ciências Biológicas, Faculdade de Ciênciasa, Farmacêutivas, UNESP, Araraquara, São Paulo, Brazil.
出版信息
Med Vet Entomol. 2006 Dec;20(4):353-7. doi: 10.1111/j.1365-2915.2006.00641.x.
The polymerase chain reaction-restriction fragment length polymorphism technique (PCR-RFLP) was used to compare Rhodnius domesticus (Neiva & Pinto), R. pictipes (Stal), R. prolixus (Stal) and R. stali (Lent, Jurberg & Galvão) (Hemiptera: Reduviidae). The enzyme BstUI differentiated R. domesticus, R. pictipes and R. prolixus, and HhaI differentiated R. domesticus, R. pictipes and R. stali. With the fingerprinting analysis generated by these two enzymes, it was possible to clearly identify all four species in the study.
采用聚合酶链反应-限制性片段长度多态性技术（PCR-RFLP）对家栖红猎蝽（Neiva & Pinto）、饰纹红猎蝽（Stal）、长红猎蝽（Stal）和斯氏红猎蝽（Lent、Jurberg & Galvão）（半翅目：猎蝽科）进行比较。BstUI酶可区分家栖红猎蝽、饰纹红猎蝽和长红猎蝽，HhaI酶可区分家栖红猎蝽、饰纹红猎蝽和斯氏红猎蝽。通过这两种酶产生的指纹图谱分析，能够清晰地鉴定出研究中的所有四个物种。
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