通过唾液血红素蛋白电泳鉴定形态相似的红猎蝽属物种（半翅目：猎蝽科：锥蝽亚科）

Identification of morphologically similar Rhodnius species (Hemiptera: Reduviidae: Triatominae) by electrophoresis of salivary heme proteins.

作者信息

Soares R P, Sant'Anna M R, Gontijo N F, Romanha A J, Diotaiuti L, Pereira M H

机构信息

Departamento de Parasitologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.

出版信息

Am J Trop Med Hyg. 2000 Jan;62(1):157-61. doi: 10.4269/ajtmh.2000.62.157.

DOI:10.4269/ajtmh.2000.62.157

PMID:10761743

Abstract

We examined intraspecific variability in the genus Rhodnius using starch gel electrophoresis of salivary heme proteins. Salivary protein profiles of 8 Rhodnius species (R. prolixus, R. robustus, R. neglectus, R. nasutus, R. ecuadoriensis, R. pallescens, R. pictipes, and R. domesticus) were compared. All species could be distinguished by this technique. The greatest protein polymorphism was found in R. ecuadoriensis, R. nasutus, R. robustus, and R. pictipes, followed by R. prolixus, R. neglectus, R. pallescens, and R. domesticus. This approach was able to distinguish R. prolixus from R. robustus and R. neglectus from R. nasutus, species with extreme phenotypical similarity.

摘要

我们使用唾液血红素蛋白的淀粉凝胶电泳研究了红猎蝽属的种内变异性。比较了8种红猎蝽（长红猎蝽、粗壮红猎蝽、忽视红猎蝽、鼻红猎蝽、厄瓜多尔红猎蝽、苍白红猎蝽、花斑红猎蝽和家居红猎蝽）的唾液蛋白谱。所有物种都可以通过这种技术区分开来。在厄瓜多尔红猎蝽、鼻红猎蝽、粗壮红猎蝽和花斑红猎蝽中发现了最大的蛋白质多态性，其次是长红猎蝽、忽视红猎蝽、苍白红猎蝽和家居红猎蝽。这种方法能够区分表型极为相似的长红猎蝽与粗壮红猎蝽，以及忽视红猎蝽与鼻红猎蝽。