编码人 A 型内皮素受体的 cDNA 的克隆与特性分析

Cloning and characterization of cDNA encoding human A-type endothelin receptor.

作者信息

Adachi M, Yang Y Y, Furuichi Y, Miyamoto C

机构信息

Department of Molecular Genetics, Nippon Roche Research Center, Kamakura, Japan.

出版信息

Biochem Biophys Res Commun. 1991 Nov 14;180(3):1265-72. doi: 10.1016/s0006-291x(05)81332-4.

DOI:10.1016/s0006-291x(05)81332-4

PMID:1719979

Abstract

A cDNA coding for the human A-type endothelin receptor (ETA) was cloned from a human placenta cDNA library. The cDNA contained the entire coding sequence for the 427 amino acid protein with a relative Mr of 48,722. The deduced amino acid sequence of the human ETA was, respectively, 94% and 93% homologous with the sequence of bovine ETA and rat ETA, but was only 64% homologous with that of the human ETB receptor. Upon expression in COS-1 cells, the human ETA receptor showed binding activity to ETA, with the highest selectivity to ET-1. Northern blot analysis showed that the mRNA of human placenta ETA consists of one species 5 kilo-nucleotides in length, and the same analysis for the uterus, testis, heart and adrenal gland of Cynomolgus monkey showed that the cognate mRNAs are widely distributed.

摘要

从人胎盘cDNA文库中克隆出编码人A型内皮素受体（ETA）的cDNA。该cDNA包含427个氨基酸蛋白质的完整编码序列，相对分子质量为48,722。推导的人ETA氨基酸序列与牛ETA和大鼠ETA序列的同源性分别为94%和93%，但与人ETB受体序列的同源性仅为64%。在COS-1细胞中表达时，人ETA受体显示出对ETA的结合活性，对ET-1的选择性最高。Northern印迹分析表明，人胎盘ETA的mRNA由一种长度为5千个核苷酸的物种组成，对食蟹猴的子宫、睾丸、心脏和肾上腺进行的相同分析表明，同源mRNA广泛分布。