Chakraborty Niranjan, Ohta Masaru, Zhu Jian-Kang
National Centre for Plant Genome Research Aruna Asaf Ali Marg JNU Campus, New Delhi, India.
Methods Mol Biol. 2007;365:287-98. doi: 10.1385/1-59745-267-X:287.
Protein phosphatase 2Cs (PP2Cs) constitute a major class of phosphatases in plants. PP2Cs play important roles in many signaling pathways by countering the action of specific protein kinases. In addition to their role in several environmental stress-related signal transduction pathways, they are also involved in plant metabolism. Protein phosphatases often physically associate with their protein kinase counterparts. One approach to understanding PP2C function is to identify their interacting protein kinases. We describe a yeast two-hybrid assay system used in our lab to determine the interaction between members of the PP2C family and protein kinases in the SOS2 family. This chapter and the cited articles describing related work might be of help in discovering interactions between other protein phosphatases and kinases.
蛋白磷酸酶2C（PP2C）构成了植物中一类主要的磷酸酶。PP2C通过对抗特定蛋白激酶的作用在许多信号通路中发挥重要作用。除了在几种与环境胁迫相关的信号转导通路中的作用外，它们还参与植物代谢。蛋白磷酸酶通常与其对应的蛋白激酶在物理上相互关联。理解PP2C功能的一种方法是确定与其相互作用的蛋白激酶。我们描述了在我们实验室中使用的酵母双杂交检测系统，以确定PP2C家族成员与SOS2家族蛋白激酶之间的相互作用。本章以及描述相关工作的引用文章可能有助于发现其他蛋白磷酸酶和激酶之间的相互作用。
