Stewart Jelena, Ware Jeffrey, Boysen Cecilie, Gulati Sandeep, Zhou Zhaozong, Rosenfeld Simon, Kopelovich Levy, Kennedy Ann R
Department of Radiation Oncology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6072, USA.
Nutr Cancer. 2008;60(6):826-36. doi: 10.1080/01635580802090193.
We previously characterized three cell clones that were derived by limiting dilution from a human prostate cancer cell line (LNCaP) representing a phenotypic continuum of cancer progression (1). The present study was undertaken to examine the effects of L-selenomethionine (SeM), a potential cancer chemopreventive agent, on the gene expression profile of the cultured cell clones. Following a three-day incubation period with SeM, total RNA was extracted, and the gene expression profile was evaluated using Affymetrix human HG U133A microarrays and analyzed by ViaLogy's (Altadena, CA) VMAxS platform deploying quantum resonance interferometry (QRI) processing. The differentially expressed genes and corresponding biological processes were compared across the different treatments and cell types. Whereas SeM significantly affected RNA-DNA metabolism and protein transport and metabolism in all of the cell types evaluated, significant effects of SeM on genes mainly involved in the pathways of cell cycle, growth, differentiation, and apoptosis were observed only in the cell clone with a more malignant phenotype.
我们之前鉴定了三个细胞克隆，它们是通过有限稀释法从代表癌症进展表型连续体的人前列腺癌细胞系（LNCaP）中获得的（1）。本研究旨在检测潜在的癌症化学预防剂L-硒代蛋氨酸（SeM）对培养的细胞克隆基因表达谱的影响。在用SeM孵育三天后，提取总RNA，并使用Affymetrix人类HG U133A微阵列评估基因表达谱，并通过部署量子共振干涉测量（QRI）处理的ViaLogy公司（加利福尼亚州阿尔塔迪纳）的VMAxS平台进行分析。比较了不同处理和细胞类型之间差异表达的基因及相应的生物学过程。虽然SeM在所有评估的细胞类型中均显著影响RNA-DNA代谢以及蛋白质转运和代谢，但仅在具有更恶性表型的细胞克隆中观察到SeM对主要参与细胞周期、生长、分化和凋亡途径的基因有显著影响。
