通过头颈癌对髓样树突状细胞中Toll样受体的干扰
Toll-like receptor interference in myeloid dendritic cells through head and neck cancer.
作者信息
Frenzel Henning, Hoffmann Bettina, Brocks Carsten, Schlenke Peter, Pries Ralph, Wollenberg Barbara
机构信息
Department of Otorhinolaryngology, University of Schleswig-Holstein Campus Liibeck, 23538 Lübeck, Germany.
出版信息
Anticancer Res. 2006 Nov-Dec;26(6B):4409-13.
BACKGROUND
In head and neck squamous cell carcinoma (HNSCC) the secretion of various immunosuppressive mediators contributes to large-scale effects on the immune functions. The influence of HNSCC on the Toll-like receptor (TLR) expression profiles of human myeloid dendritic cells (MDCs), which have been identified in human solid tumor tissue of the head and neck, was analysed.
MATERIALS AND METHODS
MDCs were isolated from peripheral blood by 'magnetic bead separation' and subsequently incubated with supernatants of HNSCC permanent cell lines. TLR expression profiles were investigated using Af cytometry and SDS-PAGE.
RESULTS
Human MDC from peripheral blood were found to express all human TLRs except TLR4 and TLR9. Incubation of MDC with supernatants of HNSCC resulted in an increased expression of TLR7.
CONCLUSION
The data suggest an interference of TLR7 expression through HNSCC and provide novel evidence of TLR alterations as a potential tumor promoting event in head and neck cancer.
背景
在头颈部鳞状细胞癌（HNSCC）中，各种免疫抑制介质的分泌对免疫功能产生大规模影响。分析了HNSCC对人头颈部实体瘤组织中已鉴定的人髓样树突状细胞（MDCs）的Toll样受体（TLR）表达谱的影响。
材料与方法
通过“磁珠分离”从外周血中分离出MDCs，随后与HNSCC永久细胞系的上清液孵育。使用流式细胞术和SDS-PAGE研究TLR表达谱。
结果
发现来自外周血的人MDCs表达除TLR4和TLR9之外的所有人TLR。MDC与HNSCC上清液孵育导致TLR7表达增加。
结论
数据表明HNSCC对TLR7表达有干扰，并提供了TLR改变作为头颈部癌症潜在肿瘤促进事件的新证据。
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