Brocks Carsten P, Pries Ralph, Frenzel Henning, Ernst Martin, Schlenke Peter, Wollenberg Barbara
Department of Otorhinolaryngology, University of Schleswig-Holstein Campus Lübeck, 23538 Lübeck, Germany.
Anticancer Res. 2007 Mar-Apr;27(2):817-24.
In head and neck squamous cell carcinoma (HNSCC), the secretion of various immunosuppressive mediators contributes to large-scale effects on the immune functions. The influence of HNSCC on various cellular functions of human myeloid dendritic cells (MDC) was analyzed in this work.
MDCs were isolated from peripheral blood by 'magnetic bead separation'. Cellular functions were analyzed using flow cytometry, migration- and ELISPOT assays as well as cytokine detection assays.
HNSCC massively affects MDCs to induce effective T-cell responses. Analysis of MDC migration and cytokine secretion revealed that HNSCC triggers the production of tumor-promoting and immunosuppressive cytokines IL-1 and IL-10 and results in an increased MDC migration activity. CpG-ODN is able to act contradictory to HNSCC.
HNSCC is able to modulate various functions of human MDCs. CpG-ODN is suggested as a potential immunostimulatory agent of human MDC.
在头颈部鳞状细胞癌（HNSCC）中，多种免疫抑制介质的分泌对免疫功能产生大规模影响。本研究分析了HNSCC对人髓样树突状细胞（MDC）各种细胞功能的影响。
通过“磁珠分离”从外周血中分离MDC。使用流式细胞术、迁移和ELISPOT分析以及细胞因子检测分析细胞功能。
HNSCC对MDC有显著影响，可诱导有效的T细胞反应。对MDC迁移和细胞因子分泌的分析表明，HNSCC触发促肿瘤和免疫抑制细胞因子IL-1和IL-10的产生，并导致MDC迁移活性增加。CpG-ODN的作用与HNSCC相反。
HNSCC能够调节人MDC的多种功能。CpG-ODN被认为是一种潜在的人MDC免疫刺激剂。
