Frenzel Henning, Pries Ralph, Brocks Carsten P, Jabs Wolfram J, Wittkopf Nadine, Wollenberg Barbara
Department of Otorhinolaryngology, Division of Nephrology, University of Schleswig-Holstein Campus Lübeck, 23538 Lübeck, Germany.
Anticancer Res. 2007 Nov-Dec;27(6B):4111-5.
In head and neck squamous cell carcinoma (HNSCC), a variety of immunomodulatory mediators contribute to strongly impaired immune functions. The secretion of C-reactive protein (CRP) by HNSCC cells and its influence on human myeloid dendritic cells (MDC) was investigated.
The CRP levels were analyzed using photometric methods and real-time PCR. The MDC were isolated from peripheral blood by 'magnetic bead separation' and incubated with different CRP concentrations. The CRP isoforms were analyzed by native PAGE (polyacrylamide gel electrophoresis). The cells were analyzed using migration assays and flow cytometry.
HNSCC cell lines were able to autonomously express C-reactive protein. Pentameric CRP triggered the down-regulation of chemokine receptor CCR5 and led to a decreased migration of human MDC.
CRP appeared to be a modulator of the migration activity of human MDC. The functional modulation of immune cells represents a crucial immune escape mechanism of human carcinomas.
在头颈部鳞状细胞癌（HNSCC）中，多种免疫调节介质导致免疫功能严重受损。研究了HNSCC细胞分泌C反应蛋白（CRP）及其对人髓样树突状细胞（MDC）的影响。
采用光度法和实时PCR分析CRP水平。通过“磁珠分离”从外周血中分离MDC，并与不同浓度的CRP孵育。通过非变性聚丙烯酰胺凝胶电泳（native PAGE）分析CRP亚型。使用迁移试验和流式细胞术分析细胞。
HNSCC细胞系能够自主表达C反应蛋白。五聚体CRP触发趋化因子受体CCR5的下调，并导致人MDC迁移减少。
CRP似乎是人MDC迁移活性的调节剂。免疫细胞的功能调节是人类癌症关键的免疫逃逸机制。
