Skurlova Martina, Stofkova Andrea, Jurcovicova Jana
Institue of Normal, Clinical and Pathological Physiology, Third Medical Faculty, Charles University, 120 00 Prague, Czech Republic.
Endocr Regul. 2006 Dec;40(4):125-8.
Proinflammatory cytokines IL-1beta, and IL-6 are synthesized in the brain, where they exert local regulatory functions. Our aim was to find out whether, along with the activation of hypothalamo-pituitary-adrenocortical (HPA) axis and prolactin (PRL), the acute systemic enhancement of IL-1beta affects its own production in the hypothalamus as well as that of IL-6.
Forty five minutes after a single i.p. administration of recombinant rat IL-1beta (5 microg/kg) to male Long Evans rats we estimated the expression of IL-1beta and IL-6 mRNA in the hypothalamus by real time PCR, ACTH, corticosterone (CORT), and PRL by RIA RESULTS: IL-1beta administration stimulated the expression of IL-1beta mRNA in the hypothalamus by 99 %, but not that of IL-6. It also significantly activated plasma levels of ACTH, PRL, CORT, and CORT production in adrenal gland.
These results indicate that acute peripheral enhancement of IL-1beta may induce neuroendocrine changes also via the immediate activation of its own expression in the hypothalamus, but not that of IL-6 expression in the hypothalamus was found.
促炎细胞因子白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）在脑内合成，在脑内发挥局部调节功能。我们的目的是探究，除了下丘脑-垂体-肾上腺皮质（HPA）轴和催乳素（PRL）的激活外，IL-1β的急性全身增强是否会影响其在下丘脑自身的产生以及IL-6的产生。
对雄性长爪沙鼠单次腹腔注射重组大鼠IL-1β（5微克/千克）45分钟后，我们通过实时聚合酶链反应（PCR）评估下丘脑内IL-1β和IL-6信使核糖核酸（mRNA）的表达，通过放射免疫分析（RIA）评估促肾上腺皮质激素（ACTH）、皮质酮（CORT）和PRL。
注射IL-1β刺激下丘脑内IL-1β mRNA的表达增加99%，但未刺激IL-6 mRNA的表达增加。它还显著激活了血浆中ACTH、PRL、CORT的水平以及肾上腺中CORT的产生。
这些结果表明，IL-1β的急性外周增强可能也通过下丘脑内其自身表达的立即激活诱导神经内分泌变化，但未发现其能激活下丘脑内IL-6的表达。
