Rasimick Brian J, Shah Rinal P, Musikant Barry L, Deutsch Allan S
Essential Dental Systems, South Hackensack, New Jersey 07606, USA.
Aust Endod J. 2010 Aug;36(2):70-3. doi: 10.1111/j.1747-4477.2009.00193.x.
Certain irrigants leave antimicrobial residues on dentine. This study assessed if residues from MTAD, Endo-CHX, Peridex Oral Rinse or 6% NaOCl were inactivated by exposure to simulated leakage. Extracted teeth (15/group) were cut and drilled to produce a cylindrical canal space. They were irrigated, dried with paper points and transferred into 1 mL of simulated leakage (Enterococcus faecalis in tryptic soy broth) every 24 h. After one, two or three transfers, the number of colony forming units was measured by drilling the canal space and recovering the dentine shavings in antimicrobial-inactivating broth. Two-way anova of the log-transformed data showed irrigation method, leakage volume and their interaction were significant factors (P < 0.001). Compared with the positive control, the logarithmic reductions in the number of E. faecalis colonies after the first and third millilitre of leakage were: Endo-CHX 6.2 and 2.4; MTAD 3.4 and 2.7; Peridex 1.6 and 0.4; NaOCl 1.0 and 0.0.
某些冲洗剂会在牙本质上留下抗菌残留物。本研究评估了MTAD、Endo-CHX、派丽奥口腔含漱液或6%次氯酸钠的残留物在暴露于模拟渗漏后是否失活。将拔除的牙齿（每组15颗）切割并钻孔以形成圆柱形根管空间。每隔24小时对根管进行冲洗，用纸尖吸干，并转移到1毫升模拟渗漏液（胰蛋白胨大豆肉汤中的粪肠球菌）中。经过一、二或三次转移后，通过钻取根管空间并在抗菌失活肉汤中回收牙本质碎屑来测量菌落形成单位的数量。对对数转换后的数据进行双向方差分析显示，冲洗方法、渗漏量及其相互作用是显著因素（P < 0.001）。与阳性对照相比，在第一毫升和第三毫升渗漏后粪肠球菌菌落数量的对数减少量分别为：Endo-CHX 6.2和2.4；MTAD 3.4和2.7；派丽奥1.6和0.4；次氯酸钠1.0和0.0。
