Dejsirilert S, Butraporn R, Chiewsilp D, Kondo E, Kanai K
National Institute of Health, Soi Bamrasnaradura Hospital, Nonthaburi, Thailand.
Jpn J Med Sci Biol. 1989 Apr;42(2):39-49. doi: 10.7883/yoken1952.42.39.
Phosphatase activities were compared quantitatively among selected species of pseudomonads. P. pseudomallei showed the highest activity of a bell-shaped pH pattern with a peak at around pH 5.0. P. cepacia had a similar pattern of milder intensity. In contrast, P. aeruginosa revealed an alkaline phosphatase activity with a pH optimum higher than 8.0, but the level of activity was much lower than those of the above two species. The enzymatic reactions of other species were slight or negligible at their optimum pH in the same test system. These data were discussed in reference to their growth behavior in different pH environments and also in connection with such recent information that the high activity of microbial acid phosphatase may be a favorable attribute to their intracellular parasitism.
对所选假单胞菌属物种的磷酸酶活性进行了定量比较。类鼻疽假单胞菌表现出最高的活性，其pH模式呈钟形，在pH 5.0左右达到峰值。洋葱伯克霍尔德菌有类似的模式，但强度较弱。相比之下，铜绿假单胞菌显示出碱性磷酸酶活性，最适pH高于8.0，但活性水平远低于上述两个物种。在相同的测试系统中，其他物种在其最适pH下的酶促反应很轻微或可忽略不计。结合它们在不同pH环境中的生长行为，以及最近有关微生物酸性磷酸酶的高活性可能是其细胞内寄生的有利属性的信息，对这些数据进行了讨论。
