Kovarík A, Hlubinová K, Prachar J, Simkovic D, Cebecauer L, Feldsamová A
Cancer Research Institute, Slovak Academy of Sciences, Bratislava, CSFR.
Neoplasma. 1991;38(5):475-82.
To study the possible involvement of a murine leukemia virus (MuLV) related agent in human cancer, an extensive immunoblotting analysis of human sera (cancer, autoimmune as well as control normal ones) for the presence of antibodies to MuLV structural proteins was performed. Out of 350 sera, 89 reacted with gag precursor Pr65, 72 reacted with major viral core protein p30 and five with the matrix protein p15. Antibody reactivity to the env-encoded glycoprotein gp70 was detected in 7 cases out of 16 sera tested. There were no significant differences between pathological and normal sera concerning the patterns and the frequency of the reactivity. Sera from patients with various malignancies (mainly with breast cancer) generally displayed more intensive signals to MuLV p30 than normal sera. Epitope mapping revealed that MuLV p30-reactive antibodies recognize an antigenic determinant(s) located at the carboxyterminus of the protein.
为研究一种鼠白血病病毒（MuLV）相关因子在人类癌症中的可能作用，对人类血清（癌症患者血清、自身免疫性疾病患者血清以及对照正常血清）进行了广泛的免疫印迹分析，以检测抗MuLV结构蛋白抗体的存在情况。在350份血清中，89份与gag前体Pr65发生反应，72份与主要病毒核心蛋白p30发生反应，5份与基质蛋白p15发生反应。在16份检测血清中的7份中检测到了针对env编码糖蛋白gp70的抗体反应性。在反应模式和频率方面，病理血清与正常血清之间没有显著差异。来自各种恶性肿瘤患者（主要是乳腺癌患者）的血清对MuLV p30通常显示出比正常血清更强的信号。表位作图显示，MuLV p30反应性抗体识别位于该蛋白羧基末端的一个或多个抗原决定簇。
