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[孤雌生殖小鼠胚胎中期染色体嗜银核仁组织区的细胞学研究]

[Cytological research on the argentophilic nucleolus-organizer regions of the metaphase chromosomes in parthenogenetic mouse embryos].

作者信息

Dyban A P, Severova E L, Noniashvili E M

出版信息

Ontogenez. 1991 Sep-Oct;22(5):525-32.

PMID:1720232
Abstract

Silver staining technique visualizing argentophilic nucleolus organizer regions (Ag-NORs) was used for studying parthenogenetic mouse embryos produced by artificial activation of oocytes in Ca(2+)-Mg(2+)-free medium. Ag-NOR-containing chromosomes were detected in metaphases of parthenogenetic embryos during six successive cleavage divisions starting with the two-cell stage. The frequency of metaphases with varying AG-NOR number in diploid parthenogenones was similar to that in the control (fertilized) embryos. Average number of metaphase Ag-NOR chromosomes (calculated per diploid chromosome set) in haploid parthenogenones exceeded that in the control; in some cases all NORs were stained by silver. This is evidence that latent ribosomal cistrons in some chromosomes can be activated.

摘要

利用银染技术观察嗜银核仁组织区(Ag-NORs),研究在无钙镁培养基中人工激活卵母细胞产生的孤雌生殖小鼠胚胎。从二细胞期开始的连续六次卵裂过程中,在孤雌生殖胚胎的中期检测到含Ag-NOR的染色体。二倍体孤雌生殖体中具有不同Ag-NOR数量的中期频率与对照(受精)胚胎相似。单倍体孤雌生殖体中期Ag-NOR染色体的平均数量(按二倍体染色体组计算)超过对照;在某些情况下,所有NORs都被银染色。这证明某些染色体中的潜在核糖体顺反子可以被激活。

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