Dyban A P, Severova E L, Zatsepina O V, Chentsov Iu S
Ontogenez. 1989 Mar-Apr;20(2):149-57.
Localization of argentophilic proteins on mouse one-cell embryos has been studied under light and electron microscope using the silver staining that reveals transcriptionally active nucleolus-organizing chromosome regions (NORs). Although argentophilic proteins are observed in NORs of metaphase chromosomes only after the second cleavage division, they can be detected in nuclear apparatus of one-cell embryos, that is at the stage when ribosomal genes are not yet transcribed. Argentophilic proteins are visualized on the surface of decondensing chromatin of the spermatozoon head and on maternal telophase chromosomes immediately after the fertilization. They migrate into pronuclei and are accumulated in pronucleoli. During mitosis argentophilic proteins bind to chromosomes and move to cytoplasm. The importance of argentophilic proteins at the initial stages of mammalian embryogenesis is discussed.
利用银染法在光学显微镜和电子显微镜下研究了嗜银蛋白在小鼠单细胞胚胎上的定位，该方法可揭示转录活跃的核仁组织染色体区域（NORs）。尽管嗜银蛋白仅在第二次卵裂后才在中期染色体的NORs中观察到，但它们可在单细胞胚胎的核装置中检测到，即核糖体基因尚未转录的阶段。受精后，嗜银蛋白立即出现在精子头部解聚染色质的表面以及母本末期染色体上。它们迁移到原核中并积聚在原核仁中。在有丝分裂期间，嗜银蛋白与染色体结合并移动到细胞质中。本文讨论了嗜银蛋白在哺乳动物胚胎发育初始阶段的重要性。
