Hackbarth H, Gwinner W, Alt J M, Hagemann I, Thiemann A, Finke B
Institut für Versuchstierkunde, Medizinische Hochschule Hannover, BRD.
Ren Physiol Biochem. 1991 Nov-Dec;14(6):246-52. doi: 10.1159/000173412.
Rats with a high number of superficial nephrons (MWF/Ztm) also show an elevated urinary protein excretion and a high systolic blood pressure. To investigate a possible correlation between the number of superficial glomeruli and these physiological changes, MWF/Ztm rats were crossed and backcrossed to Wistar cryptorchic (WC/Ztm) animals with no superficial nephrons in order to produce genotypes with differing numbers of superficial glomeruli. In the parental strains, the F1 hybrids and the 8 possible backcrosses, the number of superficial glomeruli, the distance of the 10 most superficial glomeruli to the renal surface, and the diameter of Bowman's capsules were determined by morphometric analysis. The excretion of total protein, in detail low molecular weight proteins, albumin, and high molecular weight proteins were measured quantitatively in 5 males of each genotype. Systolic blood pressure was determined by a tail-cuff method in conscious rats. Means of each variate of the 12 available genotypes were linearly correlated and demonstrate a close correlation between the amount of superficial nephrons and the observed physiological changes, i.e. the more superficial the glomeruli the higher the urinary protein excretion, especially albumin, and the higher the systolic blood pressure.
具有大量浅表肾单位的大鼠（MWF/Ztm）也表现出尿蛋白排泄增加和收缩压升高。为了研究浅表肾小球数量与这些生理变化之间的可能相关性，将MWF/Ztm大鼠与无浅表肾单位的Wistar隐睾大鼠（WC/Ztm）进行杂交和回交，以产生具有不同数量浅表肾小球的基因型。在亲本品系、F1杂种和8种可能的回交后代中，通过形态计量分析确定浅表肾小球的数量、10个最浅表肾小球到肾表面的距离以及鲍曼囊的直径。对每种基因型的5只雄性大鼠定量测量总蛋白、特别是低分子量蛋白、白蛋白和高分子量蛋白的排泄量。通过尾套法在清醒大鼠中测定收缩压。对12种可用基因型的每个变量的均值进行线性相关分析，结果表明浅表肾单位数量与观察到的生理变化之间存在密切相关性，即肾小球越浅表，尿蛋白排泄量越高，尤其是白蛋白，收缩压也越高。
