Rovira-Halbach G, Alt J M, Brunkhorst R, Frei U, Kühn K, Stolte H
Ren Physiol. 1986;9(6):317-25. doi: 10.1159/000173097.
Alterations in glomerular permeability were studied in female MWF/Ztm rats, newly selected from the Munich Wistar rats with a high number of superficial glomeruli. This strain is characterized by a diminished number of functioning nephrons, an elevated arterial blood pressure and a high proteinuria averaging 24 mg/24 h. Samples of Bowman capsular space (BCS) obtained by free-flow renal micropuncture techniques with pressure control were analyzed for albumin and high-molecular-weight (HMW) proteins by ultramicrodisc electrophoresis. The measured mean albumin and HMW protein concentration in BCS were 1.72 g/l and 0.66 g/l, respectively. The glomerular filtration rate (GFR) averaged 0.74 ml/min/g kidney weight and the single nephron GFR (SNGFR) 48 nl/min. Ultrastructural studies revealed focal bleb-like alterations of the podocytes as has been observed in hyperfiltration states. We suggest that the reduced number of functioning nephrons in the kidneys of these rats could induce glomerular hyperfiltration and permselectivity changes, which cause a high glomerular protein leakage.
在从具有大量浅表肾小球的慕尼黑Wistar大鼠中新选出的雌性MWF/Ztm大鼠中，研究了肾小球通透性的改变。该品系的特征是有功能的肾单位数量减少、动脉血压升高以及平均蛋白尿高达24mg/24小时。通过具有压力控制的自由流肾微穿刺技术获得的鲍曼囊腔（BCS）样本，采用超微盘电泳法分析白蛋白和高分子量（HMW）蛋白质。测得的BCS中白蛋白和HMW蛋白质的平均浓度分别为1.72g/l和0.66g/l。肾小球滤过率（GFR）平均为0.74ml/min/g肾重，单个肾单位GFR（SNGFR）为48nl/min。超微结构研究显示足细胞出现局灶性泡状改变，这与在超滤状态下观察到的情况相同。我们认为，这些大鼠肾脏中有功能的肾单位数量减少可能会导致肾小球超滤和选择通透性改变，进而引起高肾小球蛋白渗漏。
