Cre/loxP介导的小鼠基因组染色体工程

Cre/loxP-mediated chromosome engineering of the mouse genome.

作者信息

Brault V, Besson V, Magnol L, Duchon A, Hérault Y

机构信息

Institut de Transgénose, IEM, UMR6812, CNRS Uni-Orléans, 3B rue de la Férollerie, 45071 Orleans 2, France.

出版信息

Handb Exp Pharmacol. 2007(178):29-48. doi: 10.1007/978-3-540-35109-2_2.

DOI:10.1007/978-3-540-35109-2_2

PMID:17203650

Abstract

Together with numerous other genome modifications, chromosome engineering offers a very powerful tool to accelerate the functional analysis of the mammalian genome. The technology, based on the Cre/loxP system, is used more and more in the scientific community in order to generate new chromosomes carrying deletions, duplications, inversions and translocations in targeted regions of interest. In this review, we will present the basic principle of the technique either in vivo or in vitro and we will briefly describe some applications to provide highly valuable genetic tools, to decipher the mammalian genome organisation and to analyze human diseases in the mouse.

摘要

与许多其他基因组修饰技术一起，染色体工程提供了一个非常强大的工具来加速哺乳动物基因组的功能分析。这项基于Cre/loxP系统的技术在科学界越来越多地被使用，以产生在感兴趣的目标区域携带缺失、重复、倒位和易位的新染色体。在这篇综述中，我们将介绍该技术在体内或体外的基本原理，并简要描述一些应用，这些应用提供了非常有价值的遗传工具，用于解读哺乳动物基因组组织和分析小鼠中的人类疾病。