Yum Soo-Young, Yoon Ki-Young, Lee Choong-Il, Lee Byeong-Chun, Jang Goo
Laboratory of Theriogenology and Biotechnology, Department of Veterinary Clinical Science, College of Veterinary Medicine and the Research Institute of Veterinary Science, Seoul National University, Seoul 08826, Korea.
Department of Biotechnology & Laboratory Animals, Shingu College, Seongnam 13174, Korea.
J Vet Sci. 2016 Sep 30;17(3):261-8. doi: 10.4142/jvs.2016.17.3.261.
Animal models, particularly pigs, have come to play an important role in translational biomedical research. There have been many pig models with genetically modifications via somatic cell nuclear transfer (SCNT). However, because most transgenic pigs have been produced by random integration to date, the necessity for more exact gene-mutated models using recombinase based conditional gene expression like mice has been raised. Currently, advanced genome-editing technologies enable us to generate specific gene-deleted and -inserted pig models. In the future, the development of pig models with gene editing technologies could be a valuable resource for biomedical research.
动物模型，尤其是猪，在转化医学研究中发挥着重要作用。通过体细胞核移植（SCNT）已经产生了许多基因修饰的猪模型。然而，由于迄今为止大多数转基因猪是通过随机整合产生的，因此人们提出了像小鼠那样使用基于重组酶的条件性基因表达来构建更精确的基因敲除模型的需求。目前，先进的基因组编辑技术使我们能够生成特定基因敲除和插入的猪模型。未来，利用基因编辑技术开发猪模型可能会成为生物医学研究的宝贵资源。
