赭曲霉NCIM-1146对活性蓝-25的生物降解作用
Biodegradation of Reactive blue-25 by Aspergillus ochraceus NCIM-1146.
作者信息
Parshetti G K, Kalme S D, Gomare S S, Govindwar S P
机构信息
Department of Biochemistry, Shivaji University, Kolhapur-416 004, India.
出版信息
Bioresour Technol. 2007 Dec;98(18):3638-42. doi: 10.1016/j.biortech.2006.11.017. Epub 2007 Jan 3.
The present study dealt with the decolorization and degradation of textile dye Reactive blue-25 (0.1gl(-1)) by mycelium of Aspergillus ochraceus NCIM-1146. Spectrophotometric and visual examinations showed that the decolorization was through fungal adsorption, followed by degradation. Shaking condition was found to be better for complete and faster adsorption (7h) and decolorization (20 days) of dye Reactive blue-25 (100mgl(-1)) as compared to static condition. Presence of glucose in medium showed faster adsorption (4h) and decolorization of dye from bound (7 days) mycelium. FTIR and GCMS analysis study revealed biodegradation of Reactive blue-25 into two metabolites phthalimide and di-isobutyl phthalate.
本研究探讨了赭曲霉NCIM-1146菌丝体对纺织染料活性蓝-25（0.1g/L）的脱色和降解作用。分光光度法和视觉检查表明，脱色是通过真菌吸附，随后进行降解。与静态条件相比，发现振荡条件更有利于活性蓝-25（100mg/L）染料的完全且更快吸附（7小时）和脱色（20天）。培养基中葡萄糖的存在显示出更快的吸附（4小时）以及结合态（7天）菌丝体上染料的脱色。傅里叶变换红外光谱（FTIR）和气相色谱-质谱联用（GCMS）分析研究表明，活性蓝-25被生物降解为两种代谢产物邻苯二甲酰亚胺和邻苯二甲酸二异丁酯。
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