Saratale Ganesh, Kalme Satish, Bhosale Sanjyot, Govindwar Sanjay
Department of Biochemistry, Shivaji University, Kolhapur, India.
J Basic Microbiol. 2007 Oct;47(5):400-5. doi: 10.1002/jobm.200710337.
The filamentous fungus Aspergillus ochraceus NCIM-1146 was found to degrade kerosene, when previously grown mycelium (96 h) was incubated in the broth containing kerosene. Higher levels of NADPH-DCIP reductase, aminopyrine N-demethylase and kerosene biodegradation activities were found to be present after the growth in potato dextrose broth for 96 h, when compared with the activities at different time intervals during the growth phase. NADPH was the preferred cofactor for enzyme activity, which was inhibited by CO, indicating cytochrome P450 mediated reactions. A significant increase in all the enzyme activities was observed when mycelium incubated for 18 h in mineral salts medium, containing cholesterol, camphor, naphthalene, 1,2-dimethoxybenzene, phenobarbital, n-hexane, kerosene or saffola oil as inducers. Acetaldehyde produced by alcohol dehydrogenase could be used as an indicator for the kerosene biodegradation.
当将先前培养96小时的丝状真菌赭曲霉NCIM-1146的菌丝体在含有煤油的肉汤中培养时，发现其能够降解煤油。与生长阶段不同时间间隔的活性相比，在马铃薯葡萄糖肉汤中生长96小时后，发现NADPH-DCIP还原酶、氨基比林N-脱甲基酶和煤油生物降解活性的水平更高。NADPH是酶活性的首选辅因子，其受到CO的抑制，表明细胞色素P450介导的反应。当菌丝体在含有胆固醇、樟脑、萘、1,2-二甲氧基苯、苯巴比妥、正己烷、煤油或红花油作为诱导剂的矿物盐培养基中培养18小时时，观察到所有酶活性均显著增加。乙醇脱氢酶产生的乙醛可用作煤油生物降解的指标。
