Carrow E W, Vujcic L K, Glass W L, Seamon K B, Rastogi S C, Hendry R M, Boulos R, Nzila N, Quinnan G V
Division of Virology, Food and Drug Administration, Bethesda, MD.
AIDS Res Hum Retroviruses. 1991 Oct;7(10):831-8. doi: 10.1089/aid.1991.7.831.
Neutralizing antibodies (NA) against HIV-1MN and HIV-1IIIB, and antibodies binding to synthetic peptides (BA) derived from the gp120 envelope V3 region principal neutralizing determinants (PND) of the HIV-1MN, HIV-1IIIB, and HIV-1Z3 virus strains were assayed in HIV-1 antibody-positive sera from the United States, Haiti, Brazil, Zaire, and Zimbabwe. The ability of soluble PND peptide to block neutralization of the corresponding virus by representative sera was also tested. In each country, NA and BA titers were highest against the HIV-1MN strain, and compared with other countries, NA and BA titers against HIV-1MN were higher in sera from the United States and Haiti. When NA titers were compared with BA titers against either HIV-1MN or HIV-1IIIB, no correlation was found for the HIV-1IIIB strain, but there was a significant correlation for HIV-1MN. Addition of the HIV-1MN strain peptide to a neutralization assay for HIV-1MN resulted in a four- to tenfold reduction in NA titers in sera from the United States, Zaire, and Brazil. The results suggest that HIV-1MN and closely related variants are prevalent in many parts of the world, and that antibodies directed against the PND account for most of the neutralizing activity in sera of infected individuals.
在来自美国、海地、巴西、扎伊尔和津巴布韦的HIV-1抗体阳性血清中，检测了针对HIV-1MN和HIV-1IIIB的中和抗体（NA），以及与源自HIV-1MN、HIV-1IIIB和HIV-1Z3病毒株gp120包膜V3区主要中和决定簇（PND）的合成肽结合的抗体（BA）。还测试了可溶性PND肽阻断代表性血清对相应病毒中和作用的能力。在每个国家，针对HIV-1MN毒株的NA和BA滴度最高，与其他国家相比，美国和海地血清中针对HIV-1MN的NA和BA滴度更高。当比较针对HIV-1MN或HIV-1IIIB的NA滴度与BA滴度时，发现针对HIV-1IIIB毒株不存在相关性，但针对HIV-1MN存在显著相关性。在针对HIV-1MN的中和试验中加入HIV-1MN毒株肽，导致美国、扎伊尔和巴西血清中的NA滴度降低了4至10倍。结果表明，HIV-1MN和密切相关的变异株在世界许多地区流行，并且针对PND的抗体占感染个体血清中大部分中和活性。
