Katzenstein D A, Vujcic L K, Latif A, Boulos R, Halsey N A, Quinn T C, Rastogi S C, Quinnan G V
Laboratory of Retrovirus Research, Food and Drug Administration, Bethesda, Maryland 20892.
J Acquir Immune Defic Syndr (1988). 1990;3(8):810-6.
Neutralizing antibodies (NAs) against four isolates of human immunodeficiency virus type 1 (HIV-1) were assayed in HIV-1 antibody positive sera from the United States, Haiti, Zimbabwe, and Zaire. Overall, there were NAs detected in 95, 81, 60, and 73% of sera with reciprocal geometric mean titers (GMTs) of 626, 23, 10, and 20, respectively, against HIV-1MN, HIV-1IIIB, HIV-1RF, and HIV-1Z3. Sera from North America had significantly higher NA titers against HIV-1MN. In each country, the highest antibody titers observed were against the MN strain. Otherwise, sera from the U.S. neutralized most strongly HIV-1IIIB, sera from Zaire neutralized most strongly HIV-1Z3, and sera from Zimbabwe had equal titers against all three viruses. The differences between countries were reflected in analyses of NA titers of subgroups classified on the basis of clinical status, indicating that the differences were not likely to be related to differences in clinical status of the patients being tested. Some of this antigenic variation is reflective of known genetic diversity, while some is not. The results suggest that undefined preserved and variable regions containing neutralization epitope(s) exist. These data do not indicate a need to define antigenic subtypes of HIV-1 at present. The existence of conserved neutralization epitope(s) may indicate the potential for broad immunogenicity of appropriately selected vaccine antigens.
在来自美国、海地、津巴布韦和扎伊尔的HIV-1抗体阳性血清中检测了针对四种1型人类免疫缺陷病毒（HIV-1）分离株的中和抗体（NA）。总体而言，分别在95%、81%、60%和73%的血清中检测到了NA，针对HIV-1MN、HIV-1IIIB、HIV-1RF和HIV-1Z3的几何平均滴度（GMT）倒数分别为626、23、10和20。来自北美的血清针对HIV-1MN的NA滴度显著更高。在每个国家，观察到的最高抗体滴度均针对MN毒株。此外，美国血清对HIV-1IIIB的中和作用最强，扎伊尔血清对HIV-1Z3的中和作用最强，津巴布韦血清对所有三种病毒的滴度相同。各国之间的差异在根据临床状态分类的亚组NA滴度分析中得到了体现，这表明这些差异不太可能与被检测患者的临床状态差异有关。这种抗原变异部分反映了已知的基因多样性，部分则并非如此。结果表明存在含有中和表位的未明确的保守和可变区域。这些数据目前并未表明需要定义HIV-1的抗原亚型。保守中和表位的存在可能表明适当选择的疫苗抗原具有广泛免疫原性的潜力。
