Windhauser M M, Kappel L C, McClure J, Hegsted M
Department of Physiology, Pharmacology and Toxicology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803.
Biol Trace Elem Res. 1991 Sep;30(3):205-17. doi: 10.1007/BF02991415.
The effects of severe, moderate, and mild copper deficiencies on cellular and humoral immunity were studied. Fifty male Sprague-Dawley rats, 5 wk of age, were fed diets containing 0.5, 2.0, 3.5, or 5.0 micrograms Cu/g for either 4 or 8 wk. Ten of the rats were fed the control diet, but were pair-fed with the 0.5-micrograms/g treatment group. All rats were immunized once with sheep red blood cells. Mean plasma-copper concentration reflected the dietary levels of copper, and ceruloplasmin activity correlated highly to plasma copper. Rats consuming suboptimal levels of copper responded differently to the deficiencies, so copper status varied among those animals. After 8 wk, cell proliferation, when stimulated by phytohemagglutinin, was dependent on the copper status of the animal. Severely deficient rats had consistently lower lymphocyte stimulation indexes for phytohemagglutinin and concanavalin A, but specific antibody response was not reduced. Immunoglobulin G (IgG) concentrations were variable for all rats, and immunoglobulin M (IgM) concentrations were lower for the severely deficient rats. Suboptimal dietary copper may alter immune function in rats, depending on the ensuing effect on copper status.
研究了严重、中度和轻度铜缺乏对细胞免疫和体液免疫的影响。选用50只5周龄的雄性斯普拉格-道利大鼠，分别喂食含0.5、2.0、3.5或5.0微克铜/克的饲料，为期4周或8周。其中10只大鼠喂食对照饲料，但与0.5微克/克处理组进行配对饲养。所有大鼠均用绵羊红细胞免疫一次。血浆铜平均浓度反映了饲料中的铜水平，铜蓝蛋白活性与血浆铜高度相关。摄入铜水平低于最佳值的大鼠对铜缺乏的反应不同，因此这些动物的铜状态各不相同。8周后，经植物血凝素刺激的细胞增殖取决于动物的铜状态。严重缺乏铜的大鼠对植物血凝素和刀豆球蛋白A的淋巴细胞刺激指数一直较低，但特异性抗体反应并未降低。所有大鼠的免疫球蛋白G（IgG）浓度各不相同，严重缺乏铜的大鼠免疫球蛋白M（IgM）浓度较低。饲料中铜水平低于最佳值可能会改变大鼠的免疫功能，具体取决于其对铜状态的后续影响。
