Mulhern S A, Koller L D
Division of Nutrition, Food and Drug Administration, Washington, DC 20204.
J Nutr. 1988 Aug;118(8):1041-7. doi: 10.1093/jn/118.8.1041.
From birth mice received diets containing copper at 0.5, 1, 2 or 6 mg/kg diet. At 8 wk of age they were killed and copper status and immune responsiveness were determined. Only the groups that received copper at 0.5 or 1 mg/kg showed signs of copper deficiency, such as reduced serum ceruloplasmin, hemoglobin, hematocrit and red blood cell counts and characteristic changes in organ pathology. Body and lymphoid organ weights were altered in the groups that received copper at 0.5 or 1 mg/kg. Males were more severely affected than females. A dose-related reduction in splenic T-cell subpopulations was noted in the 0.5 and 1 mg/kg groups. Responses to lipopolysaccharide challenge were reduced, and an increase in spontaneous cycling cells was noted in the groups receiving copper at 0.5 or 1 mg/kg. Only the group receiving copper at 0.5 mg/kg had increased stem cell activity; this increase was probably due to increased erythropoiesis to meet increased demands for red blood cells in this group. These data indicate that only groups receiving copper at 0.5 or 1 mg/kg in the diet were depleted and marginally depleted in copper, respectively, and that immune hyporesponsiveness differs between the depleted and marginally depleted groups.
从出生起，小鼠就被喂食含铜量分别为0.5、1、2或6毫克/千克饮食的饲料。在8周龄时将它们处死，并测定铜状态和免疫反应性。只有接受0.5或1毫克/千克铜的组出现铜缺乏的迹象，如血清铜蓝蛋白、血红蛋白、血细胞比容和红细胞计数降低，以及器官病理学的特征性变化。接受0.5或1毫克/千克铜的组的体重和淋巴器官重量发生了改变。雄性比雌性受影响更严重。在0.5和1毫克/千克组中，观察到脾脏T细胞亚群呈剂量相关减少。在接受0.5或1毫克/千克铜的组中，对脂多糖刺激的反应降低，并且自发循环细胞增加。只有接受0.5毫克/千克铜的组干细胞活性增加；这种增加可能是由于红细胞生成增加，以满足该组对红细胞增加的需求。这些数据表明，饮食中接受0.5或1毫克/千克铜的组分别处于铜缺乏和轻度铜缺乏状态，并且铜缺乏组和轻度铜缺乏组的免疫低反应性有所不同。
